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Plant Hormones

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1497)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Real-Time Analysis of the Apical Hook Development

    • Qiang Zhu, Petra Žádníková, Dajo Smet, Dominique Van Der Straeten, Eva Benková
    Pages 1-8
  3. Grafting with Arabidopsis thaliana

    • Charles W. Melnyk
    Pages 9-18
  4. Assaying Germination and Seedling Responses of Arabidopsis to Karrikins

    • Mark T. Waters, Gavin R. Flematti, Steven M. Smith
    Pages 29-36
  5. High-Throughput Scoring of Seed Germination

    • Wilco Ligterink, Henk W. M. Hilhorst
    Pages 57-72
  6. Histochemical Staining of β-Glucuronidase and Its Spatial Quantification

    • Chloé Béziat, Jürgen Kleine-Vehn, Elena Feraru
    Pages 73-80
  7. In Silico Methods for Cell Annotation, Quantification of Gene Expression, and Cell Geometry at Single-Cell Resolution Using 3DCellAtlas

    • Petra Stamm, Soeren Strauss, Thomas D. Montenegro-Johnson, Richard Smith, George W. Bassel
    Pages 99-123
  8. FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells

    • Alejandra Freire Rios, Tatyana Radoeva, Bert De Rybel, Dolf Weijers, Jan Willem Borst
    Pages 135-146
  9. In Vivo Identification of Plant Protein Complexes Using IP-MS/MS

    • Jos R. Wendrich, Sjef Boeren, Barbara K. Möller, Dolf Weijers, Bert De Rybel
    Pages 147-158
  10. Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons

    • Mussa Quareshy, Veselina Uzunova, Justyna M. Prusinska, Richard M. Napier
    Pages 159-191
  11. Hormone Receptor Glycosylation

    • Ulrike Vavra, Christiane Veit, Richard Strasser
    Pages 205-220
  12. Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue

    • Víctor Carrasco Loba, Stephan Pollmann
    Pages 221-229

About this book

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.

Editors and Affiliations

  • IAGZ, Universität für Bodenkultur Wien, Vienna, Austria

    Jürgen Kleine-Vehn

  • Institut für Biochemie und Biologie, Universität Potsdam, Potsdam, Germany

    Michael Sauer

Bibliographic Information

  • Book Title: Plant Hormones

  • Book Subtitle: Methods and Protocols

  • Editors: Jürgen Kleine-Vehn, Michael Sauer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6469-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6467-3Published: 18 November 2016

  • Softcover ISBN: 978-1-4939-8210-3Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6469-7Published: 16 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 288

  • Number of Illustrations: 11 b/w illustrations, 106 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access