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Non-Viral Gene Delivery Vectors

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Gabriele Candiani

    1. Polytechnic University of Milan, Milan, Italy

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1445)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Conventional Bolus Gene Delivery Vectors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications

      • Santosh K. Misra, Santanu Bhattacharya
      Pages 3-17

    3. Cationic Lipid-Based Nucleic Acid Vectors

      • Emile Jubeli, William P. D. Goldring, Michael D. Pungente
      Pages 19-32

    4. Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles

      • Sara Falsini, Sandra Ristori
      Pages 33-43

    5. Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA

      • Ana L. Barrán-Berdón, Emilio Aicart, Elena Junquera
      Pages 45-61

    6. Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes

      • Edilberto Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon Zarate et al.
      Pages 63-75

    7. Quantitative Intracellular Localization of Cationic Lipid–Nucleic Acid Nanoparticles with Fluorescence Microscopy

      • Ramsey N. Majzoub, Kai K. Ewert, Cyrus R. Safinya
      Pages 77-108

    8. Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells

      • Mario Ariatti
      Pages 109-136

    9. Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers

      • Danielle Campiol Arruda, Anne Schlegel, Pascal Bigey, Virginie Escriou
      Pages 137-148

    10. Polymer Based Gene Silencing: In Vitro Delivery of SiRNA

      • Margarida I. Simão Carlos, Andreas Schätzlein, Ijeoma Uchegbu
      Pages 149-157

    11. Polyallylamine Derivatives: Novel NonToxic Transfection Agents

      • Magdalena Wytrwal, Chantal Pichon
      Pages 159-174

    12. Biodegradable Three-Layered Micelles and Injectable Hydrogels

      • Daniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, Olivia M. Merkel
      Pages 175-185

    13. Cationic Lipid-Coated Polyplexes (Lipopolyplexes) for DNA and Small RNA Delivery

      • Alexander Ewe, Achim Aigner
      Pages 187-200

    14. Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Delivery

      • Xiaomeng Huang, Mengzi Zhang, Xinmei Wang, L. James Lee, Robert J. Lee
      Pages 201-213

  3. Stimuli-Responsive Bolus Gene Delivery Vectors

    1. Front Matter

      Pages 215-215
      Download chapter PDF

    2. Characterization and Investigation of Redox-Sensitive Liposomes for Gene Delivery

      • Daniele Pezzoli, Elena Tallarita, Elena Rosini, Gabriele Candiani
      Pages 217-233

    3. From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamides

      • Stephan Morys, Ernst Wagner, Ulrich Lächelt
      Pages 235-258

    4. Gene Delivery Method Using Photo-Responsive Poly(β-Amino Ester) as Vectors

      • Nan Zheng, Yang Liu, Jianjun Cheng
      Pages 259-267

    5. Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene Therapy

      • Junjie Li, Zengshi Zha, Zhishen Ge
      Pages 269-276
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Keywords

About this book

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

Editors and Affiliations

  • Polytechnic University of Milan, Milan, Italy

    Gabriele Candiani

Bibliographic Information

  • Book Title: Non-Viral Gene Delivery Vectors

  • Book Subtitle: Methods and Protocols

  • Editors: Gabriele Candiani

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3718-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3716-5Published: 20 July 2016

  • Softcover ISBN: 978-1-4939-8118-2Published: 30 May 2018

  • eBook ISBN: 978-1-4939-3718-9Published: 19 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 290

  • Number of Illustrations: 30 b/w illustrations, 36 illustrations in colour

  • Topics: Human Genetics

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