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Analysis of Post-Translational Modifications and Proteolysis in Neuroscience

  • Book
  • © 2016

Overview

Editors:
  1. Jennifer Elizabeth Grant

    1. Department of Biology, University of Wisconsin-Stout, Menomonie, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Hong Li

    1. Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University—New Jersey Medical School Cancer Center Newark, Newark, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 114)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Proteomic Analysis of Posttranslational Modifications in Neurobiology

    • Hongbo Gu, Matthew P. Stokes, Jeffrey C. Silva
    Pages 1-29

  3. A Boronic Acid-Based Enrichment for Site-Specific Identification of the N-glycoproteome Using MS-Based Proteomics

    • Haopeng Xiao, George X. Tang, Weixuan Chen, Ronghu Wu
    Pages 31-41

  4. Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry

    • Chenxi Jia, Christopher B. Lietz, Qing Yu, Lingjun Li
    Pages 43-53

  5. Quantitative Profiling of Reversible Cysteome Modification Under Nitrosative Stress

    • Yue-Ting Wang, Sujeewa C. Piyankarage, Gregory R. J. Thatcher
    Pages 55-72

  6. Label-Free LC-MS/MS Comparative Analysis of Protein S-Nitrosome in Synaptosomes from Wild-Type and APP Transgenic Mice

    • Monika Zaręba-Kozioł, Maciej Lalowski, Aleksandra Wysłouch-Cieszyńska
    Pages 73-96

  7. Proteomics Identification of Redox-Sensitive Nuclear Protein Targets of Human Thioredoxin 1 from SHSY-5Y Neuroblastoma Cell Line

    • Mohit Raja Jain, Changgong Wu, Qing Li, Chuanlong Cui, Huacheng Dai, Hong Li
    Pages 97-110

  8. Identification and Quantification of K63-Ubiquitinated Proteins in Neuronal Cells by High-Resolution Mass Spectrometry

    • Gustavo Monteiro Silva, Wei Wei, Sandhya Manohar, Christine Vogel
    Pages 111-125

  9. Phosphorylation Site Profiling of NG108 Cells Using Quadrupole-Orbitrap Mass Spectrometry

    • Fang-Ke Huang, Guoan Zhang, Thomas A. Neubert
    Pages 127-141

  10. Phosphoproteomics of Tyrosine Kinases in the Nervous System

    • Robert J. Chalkley, Ralph A. Bradshaw
    Pages 143-154

  11. Analysis of PINK1 and CaMKII Substrates Using Mass Spectrometry-Based Proteomics

    • Yan Li, Lesley A. Kane, Michael A. Bemben, Katherine W. Roche
    Pages 155-170

  12. Liquid Chromatography Tandem Mass Spectrometry Analysis of Tau Phosphorylation

    • Jhoana Mendoza, Georgia Dolios, Rong Wang
    Pages 171-185

  13. Identification of Protease Substrates in Complex Proteomes by iTRAQ-TAILS on a Thermo Q Exactive Instrument

    • Tobias Kockmann, Nathalie Carte, Samu Melkko, Ulrich auf dem Keller
    Pages 187-207

  14. Proteolytic Processing of Neuropeptides

    • Lloyd D. Fricker
    Pages 209-220

  15. Isotope Dilution Analysis of Myelin Basic Protein Degradation After Brain Injury

    • Andrew K. Ottens
    Pages 221-242

  16. Identification and Characterization of Protein Posttranslational Modifications by Differential Fluorescent Labeling

    • Eric D. Hamlett, Cristina Osorio, Oscar Alzate
    Pages 243-262

  17. Mass Spectrometric Detection of Detyrosination and Polyglutamylation on the C-Terminal Region of Brain Tubulin

    • Yasuko Mori, Alu Konno, Mitsutoshi Setou, Koji Ikegami
    Pages 263-273

  18. Determination of Polyhydroxybutyrate (PHB) Posttranslational Modifications of Proteins Using Mass Spectrometry

    • Tong Liu, Wei Chen, Stacey Pan, Chuanlong Cui, Hong Li, Eleonora Zakharian
    Pages 275-287

  19. Back Matter

    Pages 289-290
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Keywords

About this book

This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry. Chapters include practical information pertaining to the fundamentals of sample preparation, liquid chromatography, and tandem mass spectrometry instrumental analysis and will elucidate best practices in the interpretation of data using modern bioinformatics approaches. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Biology, University of Wisconsin-Stout, Menomonie, USA

    Jennifer Elizabeth Grant

  • Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University—New Jersey Medical School Cancer Center Newark, Newark, USA

    Hong Li

Bibliographic Information

  • Book Title: Analysis of Post-Translational Modifications and Proteolysis in Neuroscience

  • Editors: Jennifer Elizabeth Grant, Hong Li

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-3472-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3470-6Published: 09 July 2016

  • Softcover ISBN: 978-1-4939-8058-1Published: 30 May 2018

  • eBook ISBN: 978-1-4939-3472-0Published: 08 July 2016

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XV, 290

  • Number of Illustrations: 20 b/w illustrations, 29 illustrations in colour

  • Topics: Neurosciences

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