Overview
- Includes new and revised cutting-edge methods and protocols for the study of chromatin
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1288)
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Table of contents (27 protocols)
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Front Matter
Keywords
About this book
Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.Editors and Affiliations
Bibliographic Information
Book Title: Chromatin Protocols
Editors: Srikumar P. Chellappan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2474-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2473-8Published: 01 April 2015
Softcover ISBN: 978-1-4939-4406-4Published: 05 October 2016
eBook ISBN: 978-1-4939-2474-5Published: 31 March 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIV, 492
Number of Illustrations: 38 b/w illustrations, 36 illustrations in colour
Topics: Cell Biology