Protein-Protein Interactions

The second edition covers a wide range of protein-protein interaction detection topics. Protein-Protein Interactions: Methods and Applications focuses on core technological platforms used to study protein-protein interactions and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing of protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces.

Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery.

• cell-based bimolecular interaction
• drug discovery
• fluorophore labeling of test proteins
• label-free biosensors and techniques
• protein-protein interactions
• quantitative and computational analysis
• small molecule modulator identification
• tag- and antibody-based methods
• traditional radioisotope

• Department of Pharmacology and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

  Cheryl L. Meyerkord

• Department of Pharmacology, Department of Hematology & Medical Oncology, and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

  Haian Fu

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