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  • © 2015

Sepsis

Diagnostic Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1237)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiii
  2. Microbiological Diagnosis of Sepsis: The Confounding Effects of a “Gold Standard”

    • Nicasio Mancini, Roberto Burioni, Massimo Clementi
    Pages 1-4
  3. Pathophysiological Aspects of Sepsis: An Overview

    • Yong-Ming Yao, Ying-Yi Luan, Qing-Hong Zhang, Zhi-Yong Sheng
    Pages 5-15
  4. Clinical Aspects of Sepsis: An Overview

    • Giacomo Monti, Giovanni Landoni, Daiana Taddeo, Francesca Isella, Alberto Zangrillo
    Pages 17-33
  5. Technical Improvements in Culturing Blood

    • Giacomo Pardini
    Pages 35-46
  6. Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures

    • Musa Y. Hindiyeh, Gill Smollan, Shiraz Gefen-Halevi, Ella Mendelson, Nathan Keller
    Pages 97-108
  7. Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols

    • Michael G. Lorenz, Helge Mühl, Claudia Disqué
    Pages 109-119
  8. Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols

    • Michael G. Lorenz, Claudia Disqué, Helge Mühl
    Pages 121-128
  9. Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples

    • Madeleine Mai, Iris Müller, Daniela Maneg, Benedikt Lohr, Achim Haecker, Gerd Haberhausen et al.
    Pages 139-147
  10. Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview

    • Marianna Parlato, Jean-Marc Cavaillon
    Pages 149-211
  11. Host Response Biomarkers in Sepsis: The Role of Procalcitonin

    • Jean-Louis Vincent, Marc Van Nuffelen, Christophe Lelubre
    Pages 213-224
  12. Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection

    • Jérémie Lemarié, Damien Barraud, Sébastien Gibot
    Pages 225-239
  13. Host Response Biomarker in Sepsis: suPAR Detection

    • Evangelos J. Giamarellos-Bourboulis, Marianna Georgitsi
    Pages 241-246

About this book

This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic  increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.

Editors and Affiliations

  • University Vita-Salute San Raffaele, Milan, Italy

    Nicasio Mancini

Bibliographic Information

  • Book Title: Sepsis

  • Book Subtitle: Diagnostic Methods and Protocols

  • Editors: Nicasio Mancini

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1776-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1775-4Published: 15 October 2014

  • Softcover ISBN: 978-1-4939-5548-0Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1776-1Published: 15 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 262

  • Number of Illustrations: 9 b/w illustrations, 18 illustrations in colour

  • Topics: Microbiology, Pathology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access