Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1193)
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Table of contents (19 protocols)
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Front Matter
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Isolation of T Cells from the Tissues
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Front Matter
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Analysis of T Cell Function and Phenotypes
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Front Matter
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In Vivo Models of T Cell Function
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Front Matter
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Keywords
About this book
T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
Reviews
From the book reviews:
“This is a useful source of current protocols designed to capture the unique features of T helper cells with tools developed for the isolation of these cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo, and in vivo. … This book is intended for all interested in learning about protocols and methods in T helper cells. Professional laboratory scientists as well as beginners in the field of immunology will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, January, 2015)Editors and Affiliations
Bibliographic Information
Book Title: T-Helper Cells
Book Subtitle: Methods and Protocols
Editors: Ari Waisman, Burkhard Becher
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1212-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1211-7Published: 23 August 2014
Softcover ISBN: 978-1-4939-4645-7Published: 17 September 2016
eBook ISBN: 978-1-4939-1212-4Published: 23 August 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 229
Number of Illustrations: 14 b/w illustrations, 24 illustrations in colour
Topics: Immunology, Pathology