Overview
- Introduces advanced usage of affinity tags
- Provides detailed protocols ready for the lab
- Includes step-by-step detail and vital advice essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1177)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.
Editors and Affiliations
Bibliographic Information
Book Title: Protein Affinity Tags
Book Subtitle: Methods and Protocols
Editors: Richard J. Giannone, Andrew B. Dykstra
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1034-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1033-5Published: 19 June 2014
Softcover ISBN: 978-1-4939-4139-1Published: 23 August 2016
eBook ISBN: 978-1-4939-1034-2Published: 18 June 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 213
Number of Illustrations: 15 b/w illustrations, 31 illustrations in colour
Topics: Protein Science, Protein Structure