Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1174)
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Table of contents (29 protocols)
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Front Matter
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An Overview of Methods for Analyzing Vesicular Trafficking
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Front Matter
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Cell-Free and Biochemical Assays of Exocytosis and Endocytosis
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Front Matter
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Imaging and Ultrastructural Analysis of Vesicle Trafficking in Cultured Cells
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Front Matter
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Keywords
About this book
Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.
Editors and Affiliations
Bibliographic Information
Book Title: Exocytosis and Endocytosis
Editors: Andrei I. Ivanov
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0944-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-0943-8Published: 20 June 2014
Softcover ISBN: 978-1-4939-4694-5Published: 23 August 2016
eBook ISBN: 978-1-4939-0944-5Published: 19 June 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIII, 435
Number of Illustrations: 38 b/w illustrations, 55 illustrations in colour
Topics: Cell Biology, Cell Physiology