Overview
- Editors:
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Gerd P. Pfeifer
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Department of Biology, Beckman Research Institute of the City of Hope, Duarte, USA
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Table of contents (31 chapters)
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Technologies for Detection of DNA Damage
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- Régen Drouin, Shuwei Gao, Gerald P. Holmquist
Pages 37-43
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- Michael Weinfeld, Michel Liuzzi, George D. D. Jones
Pages 63-71
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- Jürgen Thomale, Jörg Engelbergs, Frank Seiler, Manfred F. Rajewsky
Pages 87-101
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- Robert J. Melamede, Yoke Wah Kow, Ivan A. Bespalov, Susan S. Wallace
Pages 103-115
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- Charles A. Smith, Philip C. Hanawalt
Pages 117-129
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- F. Michael Yakes, Yiming Chen, Bennett Van Houten
Pages 171-184
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- Gopaul Kotturi, Wolfgang C. Kusser, Barry W. Glickman
Pages 185-197
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- Silvia Tornaletti, Gerd P. Pfeifer
Pages 199-209
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- Régen Drouin, Henry Rodriguez, Gerald P. Holmquist, Steven A. Akman
Pages 211-225
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- Keith A. Grimaldi, Simon R. McAdam, John A. Hartley
Pages 227-238
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Technologies for Detection of Mutations
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Front Matter
Pages 239-239
About this book
Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity. Throughout the evolution oflife, complex DNA repair systems have developed in all living organisms to cope with this damage. Unrepaired DNA lesions can promote genetic alterations (mutations) that may be linked to an altered phenotype, and, if growth-controlling genes are involved, these mutations can lead to cell transformation and the development of malignant tumors. Proto oncogenes and tumor suppressor genes may be critical targets for DNA damaging agents. In a number of animal model systems, correlations between exposure to a carcinogen, tumor develop ment, and genetic changes in tumor DNA have been established. To understand mutagenesis processes in more detail at the molecular level, we need to know the type and frequency of DNA adducts within cells, their distribution along genes and specific DNA sequences, as well as the rates at which they are repaired. We also need to know what types of mutations are produced and which gene positions are most prone to mutagenesis. This book provides a collection of techniques that are useful in mutagenesis research. The book is divided into three parts. In Part I, methods for DNA damage and repair analysis are provided.
Editors and Affiliations
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Department of Biology, Beckman Research Institute of the City of Hope, Duarte, USA
Gerd P. Pfeifer
