The Maize Handbook

1. Front Matter

   Pages i-xxvi

   PDF

2. Development and Morphology

   1. Front Matter

      Pages 1-1

      PDF

   2. Fertilization and Embryogeny in Maize

      • William F. Sheridan, Janice K. Clark

      Pages 3-10

   3. The Maize Shoot

      • R. S. Poethig

      Pages 11-17

   4. The Maize Leaf

      • Michael Freeling, Barbara Lane

      Pages 17-28

   5. The Maize Root

      • L. Feldman

      Pages 29-37

   6. Morphology and Development of the Tassel and Ear

      • Ping-Chin Cheng, Dayakar R. Pareddy

      Pages 37-47

   7. Gametogenesis in Maize

      • Patricia Bedinger, Scott D. Russell

      Pages 48-61

   8. The Patterns of Plant Structures in Maize

      • Walton C. Galinat

      Pages 61-65

   9. Genetics and the Morphological Evolution of Maize

      • John Doebley

      Pages 66-77

   10. Overview of Key Steps in Aleurone Development

       • Virginia Walbot

       Pages 78-80

3. Cell Biology

   1. Front Matter

      Pages 81-81

      PDF

   2. Light Microscopy I: Dissection and Microtechnique

      • Anne W. Sylvester, Steven E. Ruzin

      Pages 83-95

   3. Light Microscopy II: Observation, Photomicrography, and Image Analysis

      • Steven E. Ruzin, Anne W. Sylvester

      Pages 95-108

   4. Scanning Electron Microscopy

      • Michelle H. Williams, Anne W. Sylvester

      Pages 108-117

   5. Transmission Electron Microscopy: Chemical Fixation, Freezing Methods, and Inmunolocalization

      • M. V. Parthasarathy

      Pages 118-134

   6. Techniques for Histology of Maize Megaspores and Embryo Sacs

      • S. D. Russell, D. P. West

      Pages 135-139

   7. Indirect Immunofluoreseence: Localization of the Cytoskeleton

      • C. J. Staiger

      Pages 140-149

   8. Immunocytochemistry for Light and Electron Microscopy

      • M. J. Varagona, N. V. Raikhel

      Pages 149-157

   9. Immunolocalization of Nuclear Proteins

      • Laurie G. Smith

      Pages 158-164

The Maize Handbook represents the collective efforts of the maize research community to enumerate the key steps of standard procedures and to disseminate these protocols for the common good. Although the material in this volume is drawn from experience with maize, many of the procedures, protocols, and descriptions are applicable to other higher plants, particularly to other grasses. The power and resolution of experiments with maize depend on the wide range of specialized genetic techniques and marked stocks; these materials are available today as the culmination of nearly 100 years of genetic research. A major goal of this volume is to introduce this genetical legacy and to highlight current stock construction programs that will soon benefit our work, e. g. high-density RFLP maps, deletion stocks, etc. Both stock construction and maintenance are relatively straightforward in maize as a result of the ease of crossing and the longevity of stored seeds. Crossing is facilitated by the separate staminate (tassel) and pistillate (ear) flowers, a feature almost unique to maize. On the other hand, many of the genetic methodologies utilized with maize, including the precision of record keeping, can be adapted to other plants. Facile communication and a spirit of co-operation have characterized the maize genetics community since its earliest days. Starting in the 1930s, institutions such as annual Maize Genetics Cooperation Newsletter, the Maize Genetics Stock Center, and the annual maize genetics meeting provide continuity to the field.

• biology
• development
• molecular biology
• plant
• plant genetics
• plants

"The book is certainly one that every university and research station library should buy, and every plant geneticist should have access to." Plant Genetics Newsletter
"This handbook sets the standard and is an essential work for anyone doing maize research of any type." Choice

• Department of Plant Biology, University of California, Berkeley, USA

  Michael Freeling

• Department of Biological Sciences, Stanford University, Stanford, USA

  Virginia Walbot

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