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Neural Reprogramming

Methods and Protocols

Editors:

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2352)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts

    • Juan M. Adrian-Segarra, Bettina Weigel, Moritz Mall
    Pages 1-12
  3. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons

    • Nesrin Sharif, Filippo Calzolari, Benedikt Berninger
    Pages 13-29
  4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules

    • E Tian, Mingzi Zhang, Yanhong Shi
    Pages 45-55
  5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons

    • Amel Falco, Rocío Bartolomé-Cabrero, Sergio Gascón
    Pages 57-71
  6. Direct Conversion of Human Fibroblasts to Induced Neurons

    • Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, Jerome Mertens
    Pages 73-96
  7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts

    • Emilie M. Legault, Janelle Drouin-Ouellet
    Pages 97-115
  8. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors

    • Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, Haruhisa Inoue
    Pages 127-132
  9. Transcriptional Profiling During Neural Conversion

    • Yohannes Afeworki, Hannah Wollenzien, Michael S. Kareta
    Pages 171-181
  10. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

    • Srisaiyini Kidnapillai, Daniella Rylander Ottosson
    Pages 183-199
  11. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions

    • Juan M. Adrian-Segarra, Bettina Weigel, Moritz Mall
    Pages 227-236
  12. Correction to: Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

    • Srisaiyini Kidnapillai, Daniella Rylander Ottosson
    Pages C1-C1
  13. Back Matter

    Pages 261-263

About this book

This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.

Editors and Affiliations

  • Stem Cells, Aging and Neurodegeneration Group, Faculty of Medicine, Department of Clinical Sciences, Neurology, Lund Stem Cell Center, Lund University, Lund, Sweden

    Henrik Ahlenius

Bibliographic Information

  • Book Title: Neural Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Henrik Ahlenius

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1601-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1600-0Published: 30 July 2021

  • Softcover ISBN: 978-1-0716-1603-1Published: 30 July 2022

  • eBook ISBN: 978-1-0716-1601-7Published: 29 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 263

  • Number of Illustrations: 3 b/w illustrations, 43 illustrations in colour

  • Topics: Neurosciences, Biomedical Engineering and Bioengineering

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access