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Peptide Nucleic Acids

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Peter E. Nielsen

    1. Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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  • Includes cutting-edge methods and Protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2105)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Fmoc-Based Assembly of PNA Oligomers: Manual and Microwave-Assisted Automated Synthesis

    • Ashif Yasin Shaikh, Anna Mette Hansen, Henrik Franzyk
    Pages 1-16

  3. A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers

    • Wei-Che Hsieh, Danith H. Ly
    Pages 17-33

  4. Synthesis of Pyrrolidinyl PNA and Its Site-Specific Labeling at Internal Positions by Click Chemistry

    • Boonsong Ditmangklo, Penthip Muangkaew, Kotchakorn Supabowornsathit, Tirayut Vilaivan
    Pages 35-60

  5. Synthesis of PNA-Peptide Conjugates as Functional SNARE Protein Mimetics

    • Barbara E. Hubrich, Patrick M. Menzel, Benedikt Kugler, Ulf Diederichsen
    Pages 61-74

  6. Lipid-Modified Peptide Nucleic Acids: Synthesis and Application to Programmable Liposome Fusion

    • Philipp M. G. Löffler, Alexander Rabe, Stefan Vogel
    Pages 75-96

  7. Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimide-Thioether Linkage

    • Anna Mette Hansen, Ashif Yasin Shaikh, Henrik Franzyk
    Pages 97-118

  8. PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries

    • Jacques Saarbach, Sofia Barluenga, Nicolas Winssinger
    Pages 119-139

  9. Transcription Inhibition by PNA-Induced R-Loops

    • Boris P. Belotserkovskii, Sum-yan Ng, Philip C. Hanawalt
    Pages 141-155

  10. Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA

    • Nikita Brodyagin, Dziyana Hnedzko, James A. MacKay, Eriks Rozners
    Pages 157-172

  11. In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)

    • Takehiko Shiraishi, Mahdi Ghavami, Peter E. Nielsen
    Pages 173-185

  12. Reactive Quantum Dot-Based FRET Systems for Target-Catalyzed Detection of RNA

    • Oleksandr Zavoiura, Ute Resch-Genger, Oliver Seitz
    Pages 187-198

  13. Peptide Nucleic Acids for MicroRNA Targeting

    • Roberto Gambari, Jessica Gasparello, Enrica Fabbri, Monica Borgatti, Anna Tamanini, Alessia Finotti
    Pages 199-215

  14. Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid

    • Ricardo Oliveira, Carina Almeida, Nuno F. Azevedo
    Pages 217-230

  15. PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates

    • Lise Goltermann, Peter E. Nielsen
    Pages 231-239

  16. In Vivo Administration of Splice Switching PNAs Using the mdx Mouse as a Model System

    • Camilla Brolin, Ernest Wee Kiat Lim, Peter E. Nielsen
    Pages 241-250

  17. Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

    • Ernest Wee Kiat Lim, Camilla Brolin, Peter E. Nielsen
    Pages 251-260

  18. Poly(Lactic-co-Glycolic Acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo

    • Stanley N. Oyaghire, Elias Quijano, Alexandra S. Piotrowski-Daspit, W. Mark Saltzman, Peter M. Glazer
    Pages 261-281

  19. Preparation of Conjugates for Affibody-Based PNA-Mediated Pretargeting

    • Mohamed Altai, Anzhelika Vorobyeva, Vladimir Tolmachev, Amelie Eriksson Karlström, Kristina Westerlund
    Pages 283-304

  20. Back Matter

    Pages 305-308
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Keywords

About this book

This third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book.  Chapters focus on in vivo properties and behavior and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

 

Editors and Affiliations

  • Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Peter E. Nielsen

Bibliographic Information

  • Book Title: Peptide Nucleic Acids

  • Book Subtitle: Methods and Protocols

  • Editors: Peter E. Nielsen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0243-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0242-3Published: 23 February 2020

  • Softcover ISBN: 978-1-0716-0245-4Published: 23 February 2021

  • eBook ISBN: 978-1-0716-0243-0Published: 22 February 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 308

  • Number of Illustrations: 80 b/w illustrations, 41 illustrations in colour

  • Topics: Protein Science

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