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The Spectroscopic Study of Indocyanine Green J-Aggregate Stability in Human Blood and Plasma

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Abstract

Spectroscopic approaches are very good to noninvasively determine the most significant indicators of the tissue state. Indocyanine green (ICG) is a well-known fluorescent dye approved for clinical applications, which has a short circulation time in the vascular system and low photostability. At high temperatures the molecular solution of the photosensitizer self-assembles into a stable J-aggregate form of ICG nanoparticles (ICG NPs) with the absorption peak in the near-infrared range. Investigation of ICG NP stability in human blood and plasma using a fiber-spectroscopic system demonstrates no difference in absorption properties and different dependence of the integrated fluorescence ratio between ICG monomers and J-aggregates in blood and plasma. Transition of ICG NP aggregates to the monomeric form in human blood plasma results in a higher circulation time of the fluorescent dye in the vascular system. High stability of aggregates and a low elimination rate may increase efficiency of fluorescent diagnostics of near-tumor tissues.

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Funding

The research was performed within the State Assignment for conducting research under Government contract in 2019–2021 (theme no. 0723-2020-0035 “New phenomena in interaction of laser radiation, plasma, and particle and radiation fluxes with condensed matter as a basis of innovative technologies”) and supported in part by the Russian Foundation for Basic Research, project no. 18-29-01062.

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Authors and Affiliations

  1. Prokhorov General Physics Institute of the Russian Academy of Science, 119991, Moscow, Russia

    D. S. Farrakhova, I. D. Romanishkin, D. V. Yakovlev, Yu. S. Maklygina, T. A. Savelieva & V. B. Loschenov

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, 117997, Moscow, Russia

    D. V. Yakovlev

  3. National Research Nuclear University MEPhI, 115409, Moscow, Russia

    T. A. Savelieva & V. B. Loschenov

  4. Institut de Cancérologie de Lorraine, 54519, Vandoeuvre-lès-Nancy, France

    L. Bezdetnaya

  5. Centre de Recherche en Automatique de Nancy, CNRS, Université de Lorraine, 54519, Vandoeuvre-lès-Nancy, France

    L. Bezdetnaya

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  1. D. S. Farrakhova
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  2. I. D. Romanishkin
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  3. D. V. Yakovlev
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  4. Yu. S. Maklygina
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  5. T. A. Savelieva
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  6. L. Bezdetnaya
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  7. V. B. Loschenov
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Correspondence to D. S. Farrakhova.

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Translated by M. Potapov

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Farrakhova, D.S., Romanishkin, I.D., Yakovlev, D.V. et al. The Spectroscopic Study of Indocyanine Green J-Aggregate Stability in Human Blood and Plasma. Phys. Wave Phen. 30, 86–90 (2022). https://doi.org/10.3103/S1541308X22020029

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  • DOI: https://doi.org/10.3103/S1541308X22020029

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