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Reconstruction of Action Potentials of Cardiac Cells from Extracellular Field Potentials

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Abstract

The key areas of application of systems with microelectrode arrays (MEA) are studying the mechanisms of diseases and testing the effect of drugs on the human body using “laboratory-on-a-chip” technologies based on researches of artificially grown cells. Many scientists’ efforts are directed to the processing and analysis of information received by MEA systems, helping the doctors in creating effective treatment strategies. However, field potentials (FP) of cardiac cells recorded with MEA systems in non-invasive measurements provide incomplete information for the estimation of ionic currents, compared to invasive measurements of action potentials (AP) obtained using patch-clamp technology. The research is devoted to the mathematical determination of the relationship between the signals of electrical activity of cardiomyocytes: internal AP and external FP. In this paper it is proposed a method for solving the inverse problem of the relationship between AP and FP. The equation for the transfer functions between AP and FP is obtained on the basis of field theory. The paper presents the results of AP reconstruction modeling using measured FPs, demonstrating the change in the morphology and parameters of these signals under the influence of dimethylsulfoxide (DMSO). FP signals are recorded using non-destructive electrophysiological technology based on microelectrode coaxial guides (μECG), which can be considered as a type of MEA.

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Acknowledgments

The authors express their gratitude to the employees of the Milan Polytechnic A. Redaelli and R. Visone for providing recordings of extracellular field potentials, which were registered using μECG technology.

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Authors and Affiliations

  1. National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, Ukraine

    Nataliia G. Ivanushkina, Kateryna O. Ivanko, Mykhailo O. Shpotak & Yuriy V. Prokopenko

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  1. Nataliia G. Ivanushkina
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  2. Kateryna O. Ivanko
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Correspondence to Nataliia G. Ivanushkina.

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N. G. Ivanushkina, K. O. Ivanko, M. O. Shpotak, Yu. V. Prokopenko

The authors declare that they have no conflicts of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

The initial version of this paper in Russian is published in the journal “Izvestiya Vysshikh Uchebnykh Zavedenii. Radioelektronika,” ISSN 2307-6011 (Online), ISSN 0021-3470 (Print) on the link http://radio.kpi.ua/article/view/S0021347022090047 with DOI: https://doi.org/10.20535/S0021347022090047

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Translated from Izvestiya Vysshikh Uchebnykh Zavedenii. Radioelektronika, No. 7, pp. 420-432, July, 2022 https://doi.org/10.20535/S0021347022090047 .

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Ivanushkina, N.G., Ivanko, K.O., Shpotak, M.O. et al. Reconstruction of Action Potentials of Cardiac Cells from Extracellular Field Potentials. Radioelectron.Commun.Syst. 65, 354–364 (2022). https://doi.org/10.3103/S0735272722090047

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