Abstract
Chromium picolinate is used in the poultry diet because of its antistress effects in addition to the fact that the requirement for it is increased during stress. This study was conducted to determine if the negative effects of high ambient temperature (34°C) on egg production, egg quality, antioxidant status, and cholesterol and mineral content of egg yolk could be alleviated by combination of chromium picolinate and biotin (0.6/2.0; Diachrome™, as formulated by Nutrition 21 Inc.), in laying Japanese quails (Coturnix coturnix japanica), Quails (n=240; 50 d old) were divided into 8 groups, 30 birds per group. The quails were fed either a basal diet or the basal diet supplemented with 2, 4, or 8 mg of Diachrome/kg diet. Birds were kept at 22°C and 53% relative humidity (RH). At 14 wk of age, the thermoneutral (TN) group remained in the same temperature as at the beginning of experiment, whereas the heat stress (HS) group was kept in an environment-controlled room (34°C and 41% RH) for 3 wk. Heat exposure decreased performance when the basal diet was fed (p=0.001). Diachrome supplementation at 4 and 8 mg/kg diet, increased feed intake (p=0.05), egg production (p=0.05), feed efficiency (p=0.01), egg weight (p=0.05), and Haugh unit (p=0.01) in quails reared under heat stress conditions. Heat exposure increased concentrations of serum malondialdehyde (MDA) (p=0.001), glucose, and cholesterol (p=0.01), which were elevated by supplemental Diachrome (p≤0.05). Egg yolk Cr, Zn, and Fe (p=0.01) concentrations increased linearly, whereas MDA and cholesterol concentrations decreased (p=0.05) as dietary Diachrome supplementation increased in HS groups. Similar effects of supplementation on serum levels of glucose and cholesterol (p=0.05) and egg yolk concentrations of cholesterol (p=0.05) and Cr (p=0.01) were observed in TN groups. No significant differences in other values were observed in the TN groups. Results of the present study suggest that supplementation with Diachrome protects the quail by reducing the negative effects of heat stress.
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A. J. Smith and O. Oliver, some nutritional problems associated with egg production at high environmental temperatures. I. The effect of environmental temperature and rationing treatments in the productivity of pullets fed on diets of differing energy content, Rhod. J. Agric. Res. 10, 3–20 (1972).
D. Wolfenson, Y. F. Feri, N. Snapir, and A. Berman, Effect of diurnal or nocturnal heat stress on egg formation, Br. Poult. Sci. 20, 167–74 (1979).
A. Donkoh, Ambient temperature: a factor affecting performance and physiological response of broiler chickens, Int. J. Biometeorol. 33, 259–265 (1989).
K. Sahin and O. Kucuk, Heat stress and dietary vitamin supplementation of poultry diets, Nutr. Abstr. Rev. B. Livestock Feeds Feeding, 73, 41R-50R (2003).
M. V. Borisiuk and V. Zinchuk, Analysis of the relationship between hemoglobin-oxygen affinity and lipid peroxidation during fever, Acta Biochem. Polon. 42, 69–74 (1995).
Y. Iwagami, Changes in the ultrastructure of human cells related to certain biological responses under hyperthermic culture conditions, Hum. Cell. 9, 353–366 (1996).
L. R. McDowell, Vitamins in animal nutrition, in Comparative Aspects to Human Nutrition; Vitamin C. L. R. Mc Dowell, ed., Academic, London, pp. 365–387 (1989).
D. N. Mowat, Organic chromium. A new nutrient for stressed animals, Biotechnology in the Feed Industry: Proceedings of Alltech’s Tenth Annual Symposium, T. P. Lyons and K. A. Jacques, eds., Nottingham University Press, Nottingham, UK, pp. 275–282 (1994).
K. Sahin, M. Onderci, N. Sahin, and S. Aydin, Effects of dietary chromium picolinate and ascorbic acid supplementation on egg production, egg quality and some serum metabolites of laying hens reared under a low ambient temperature (6 degrees C), Arch. Anim. Nutr. 56, 41–49 (2002).
K. Sahin, N. Sahin, M. Onderci, M. F. Gursu, and G. Cikim, Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens, Biol. Trace Element Res. 89, 53–64 (2002).
N. Sahin, M. Onderci, and K. Sahin, Effects of Dietary chromium and zinc on egg production, egg quality and some blood metabolites of laying hens reared under low ambient temperature, Biol. Trace Element Res. 85, 47–58 (2002).
R. J. Doisy, Effect of nutrient deficiencies in animals; chromium in CRC Handbook Series in Nutrition and Food. Section E: Nutritional Disorders, Vol. 2. Effect of Nutrient Deficiencies in Animals, M. Rechcigi, Jr., ed., CRC, West Palm Beach, FL, pp. 341–342 (1978).
R. A. Anderson, Trace Elements in Human and Animal Nutrition, Academic, New York, pp. 225–244 (1987).
W. Mertz, Chromium in human nutrition: a review, J. Nutr. 123, 626–633 (1993).
R. A. Anderson and A. S. Kozlovsky, Chromium intakes, absorption and excretion of subjects consuming self-selected diets, Am. J. Clin. Nutr. 41, 1171–1183 (1985).
R. W. Rosebrough and N. C. Steele, Effect of supplemental dietary chromium or nicotic acid on carbonhydrate metabolism during basal, starvation and refeeding periods in poults, Poult. Sci. 60, 407–411 (1981).
M. F. McCarty, The case for supplemental chromium and a survey of clinical studies with chromium picolinate, J. Appl. Nutr. 43, 58–66 (1991).
T. G. Page, Chromium, tryptophan and picolinate in diets for pigs and poultry, Ph.D. dissertation, Louisiana State University (1991).
D. D. Gallaher, A. S. Csallany, D. W. Shoeman, and J. M. Olson, Diabetes increases excretion of urinary malondehyde cojugates in rats, Lipids 28, 663–666 (1993).
H. G. Preuss, P. L. Grojec, S. Lieberman, and R. A. Anderson, Effects of different chromium compounds on blood pressure and lipid peroxidation in spontaneously hypertensive rats, Clin. Nephrol. 47(5), S25–30 (1997).
R. I. Press, J. Geller, and G. W. Evans, The effect of chromium picolinate on serum cholesterol and apolipoprotein fractions in human subjects, West. J. Med. 152, 41–45 (1990).
J. D. Pagan S. G. Jackson, and S. E. Duren, The effect of chromium supplementation on metabolic response to exercise in thoroughbred horses, in Biotechnology in the Feed Industry: Proceedings of Alltech’s Eleventh Annual Symposium, T. P. Lyons and K. A. Jacques, eds. Nottingham University Press, Nottingham, UK, 249–256 (1995).
R. A. Anderson, Stress effects on chromium nutrition of humans and farm animals, in Biotechnology in the Feed Industry, T. P. Lyons and K. A. Jacques, eds., Nottingham University Press, Nothingam, UK, pp. 267–274 (1994).
NRC The Role of Chromium in Animal Nutrition, National Academy Press, Washington, DC (1997).
T. Lien, S. Chen, S. Shiau, D. Froman, and C. Y. Hu, Chromium picolinate reduces laying hen serum and egg yolk cholesterol, Professional Anim. Scientist. 12, 77–80 (1996).
K. L. Chen, et al., Effect of dietary chromium nicotinate on performance, serum traits and carass characteristics of female turkeys, J. Biomass Energy Soc. China 17, 56–62 (1998).
T. F. Lien, Y. M. Horng, and K. H. Yang, Performance, serum characteristics, carcass traits and lipid metabolism of broilers as affected by supplement of chromium picolinate, Br. Poult. Sci. 40(3, 357–361 (1999).
A. J. Wright, D. N. Mowat, and B. A. Mallard, Supplemental chromium and bovine respiratory disease vaccines for stressed feeder calves, Can. J. Anim. Sci. 74, 287–293 (1994).
K. Sahin, N., Sahin, and O. Kucuk, Effects of dietary chromium and ascorbic acid supplementation of digestion of nutrients, serum antioxidant status and mineral concentrations in laying hens reared at a low ambient temperature, Biol. Trace Element Res. 87, 113–124 (2002).
K. Dakshinamurti and C. Cheah-Tan, Biotin-mediated synthesis of hepatic glucokinase in the rat, Arch. Biochem. Biophys. 127, 17–21 (1968).
L. J. Machlin, Biotin, in Handbook of Vitamins 2nd ed. revised and expanded, Marcel Dekker, New York, pp. 393–427 (1991).
Z. Q. Wang, X. H. Zhang, and W. T. Cefalu, Chromium picolinate and biotin enhance glycogen synthesis and glycogen synthase gene expression in human skeletal muscle culture, in the 17th International Diabetes Federation Congress, Mexico City, 2000.
J. R. Komorowski, J. De La Harpe, W. T. Cefalu, X. H. Zhang, Z. Q. Wang, and D. Greenberg. JCR:LA-cp rats show improved lipid profiles in response to diets containing chromium picolinate and biotin, in Meeting of the Society for the Study of Ingestive Behavior, Philadelphia, 2001.
NRC, Nutrient Requirements of Poultry, 9th rev. ed., National Academy Press, Washington, DC (1994).
E. J. Eisen, B. B. Bohren, and H. E. McKean, The haugh unit as a measure of egg albumen quality, Poult. Sci. 41, 1461–1468 (1962).
N. Sahin, K. Sahin, M. Onderci, M. Ozcelik, and M. O. Smith, In vivo antioxidant properties of vitamin E and chromium in cold-stressed Japanese quails Arch. Anim. Nutr. 57(3), 207–215 (2003).
L. P. Berrio and J. A. Hebert, The effect of adding cholesterol to laying hen diets as powder or predissolved in fat, Poult. Sci. 69, 972–976 (1990).
A. Franchini, F. Sirri, N. Tallarico, G. Minelli, N. Iaffaldano, and A. Meluzzi, Oxidative stability and sensory and functional properties of eggs from laying hens fed supranutritional doses of vitamins E and C, Poult. Sci. 81(11), 1744–1750 (2002).
AOAC, Official Methods of Analysis, 15th ed., Association of Official Analytical Chemists, Arglinton, VA (1990).
SAS Institute, SAS ® User’s Guide: Statistics SAS Institute Inc., Cary, NC (1996).
M. E. Ensminger, J. E. Oldfield, and W. W. Heinemann, Feeds and Nutrition, Ensminger Publishing, Cloyis, CA, pp. 108–110 (1990).
R. E. Austic, Feeding poultry in hot and cold climates, in Stress Physiology in Livestock, M. K. Yousef, ed., CRC, Boca Raton, FL, Vol. 3, pp. 123–136 (1985).
P. A. Geraert, J. C. F. Padilha, and S. Guillaumin, Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: growth performance, body composition and energy retention, Br. J. Nutr. 75, 195–204 (1996).
J. S. Borel, T. C. Majerus, M. M. Polansky, P. B. Moser, and R. A. Anderson, Chromium intake and urinary chromium excretion of trama patients, Biol. Trace Element Res. 6, 317–321 (1984).
Y. H. Kim, In K. Han, I. S. Shin, B. J. Chae, and T. H. Kang, Effects of dietary levels of chromium picolinate on growth performance, carcass quality and serum traits in broiler chicks, AJAS 9, 341–347 (1996).
P. X. Liu, L. J. Chen, D. B. Xie, and X. M. Xiong, Effects of dietary chromium on the productivity of laying hens and the distribution of chromium, Acta Agric. Univ. Jangxiensis 21, 564–568 (1999).
G. W. Evans, The effect of chromium picolinate on insulin controlled parameters in humans, Int. J. Biophys. Med. Res. 11, 163–180 (1989).
R. A. Anderson, M. M. Polansky, N. A. Bryden, and J. J. Canary, Supplementalchromium effects on glucose, insulin, glucagon and uninary chromium losses in subjects consuming controlled low-chromium diets, Am. J. Clin. Nutr. 54, 909–916 (1991).
M. C. Linder, Nutrition and metabolism of the trace elements, in Nutritional Biochemistry and Metabolism with Clinical Applications M. C. Linder, ed, Elsevier, New York, pp. 215–276 (1991).
M. A. Cupo and W. E. Donaldson, Chromium and vanadium effects on glucose metabolism and lipid synthesis in the chick, Poult. Sci. 66, 120–126 (1987).
S. Okado, H. Tsukada, and H. Ohba, Enhancement of nucleolar RNA synthesis by chromium(III) in regenerating rat liver, J. Inorg. Biochem. 21, 113–124 (1984).
D. C. Luadicina and L. J. Marnett, Enhancement of hydroperoxide-dependent lipid peroxidation in rat liver microsomes by ascorbic acid, Arch. Biochem. Biophys. 278, 73–80 (1990).
M. Onderci, et al., Antioxidant properties of chromium and zinc: in vivo effects on digestibility, lipid peroxidation, antioxidant vitamins, and some minerals under a low ambient temperature, Biol. Trace Element Res. 92(2), 139–149 (2003).
W. R. Beisel, Single nutrients and immunity, Am. J. Clin. Nutr. 35, 442–451 (1982).
L. S. Tufft and C. F. Nockles, The effects of stress, Escherichia coli, dietary ethylenedi-amintetraacetic acid, and their interaction on tissue trace elements in chicks, Poult. Sci. 70, 2439–2449 (1991).
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Sahin, K., Onderci, M., Sahin, N. et al. Effects of dietary combination of chromium and biotin on egg production, serum metabolites, and egg yolk mineral and cholesterol concentrations in heat-distressed laying quails. Biol Trace Elem Res 101, 181–192 (2004). https://doi.org/10.1385/BTER:101:2:181
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DOI: https://doi.org/10.1385/BTER:101:2:181