Abstract
The DNA extraction process represents one of the critical stages in the analysis of degraded or ancient DNA. If polymerase chain reaction (PCR) amplification starts from a poor extract containing low template quantities, stochastic variation in the amplification of individual alleles may lead to allelic dropout (1), resulting in a high risk of false-homozygous typing of a heterozygous sample (2).
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© 2003 Humana Press Inc.
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Schmerer, W.M. (2003). Extraction of Ancient DNA. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:57
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DOI: https://doi.org/10.1385/1-59259-384-4:57
Publisher Name: Humana Press
Print ISBN: 978-0-89603-642-0
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