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Aggregating Neural Cell Cultures

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Protocols for Neural Cell Culture

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Aggregating brain cell cultures are primary, three-dimensional cell cultures consisting of even-sized, spherical structures that are maintained in suspension by constant gyratory agitation. Because of the avidity of freshly dissociated embryonic cells to attach to their counterparts, cell aggregates form spontaneously and rapidly under appropriate culture conditions. The reaggregated cells are able to migrate within the formed structures, and to interact with each other by direct cell-cell contact, as well as through exchange of nutritional and signaling factors. This tissue-specific environment enables aggregating neural cells to differentiate, and to develop specialized structures (e.g., synapses, myelinated axons) resembling those of brain tissue in situ. Aggregating cell cultures are therefore classified as organotypic cultures (Doyle et al., 1994).

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Author information

Authors and Affiliations

  1. Institut de Physiologie, Faculté de Médecine, Universite de Lausanne, Lausanne, Switzerland

    Paul Honegger & Florianne Monnet-Tschudi

Authors
  1. Paul Honegger
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  2. Florianne Monnet-Tschudi
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Editor information

Editors and Affiliations

  1. Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Sergey Fedoroff  & Arleen Richardson  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Honegger, P., Monnet-Tschudi, F. (2001). Aggregating Neural Cell Cultures. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:199

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  • DOI: https://doi.org/10.1385/1-59259-207-4:199

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-902-5

  • Online ISBN: 978-1-59259-207-4

  • eBook Packages: Springer Book Archive

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