Abstract
A successful cloning of polymerase chain reaction (PCR)-derived DNA fragment is a key step for further analysis of the amplified DNAs, though it is often a difficult task. Many cloning methods have been established; various commercial cloning kits are also available. These methods can be separated into two groups: ligation-dependent cloning and ligation-independent cloning. The former is used more widely than the latter. According to DNA ends, the existing ligation-dependent cloning methods for PCR products can be further divided into three types: blunt-end cloning, sticky-end cloning, and T-A cloning.
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Guo, B., Bi, Y. (2002). Cloning PCR Products. In: Chen, BY., Janes, H.W. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 192. Humana Press. https://doi.org/10.1385/1-59259-177-9:111
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DOI: https://doi.org/10.1385/1-59259-177-9:111
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Print ISBN: 978-0-89603-969-8
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