Nucleic Acids

1. Front Matter

   Pages i-xiii

   PDF

2. The Burton Assay for DNA

   • Jaap H. Waterborg, Harry R. Matthews

   Pages 1-3

3. DABA Fluorescence Assay for Submicrogram Amounts of DNA

   • Theodore Gurney Jr., Elizabeth G. Gurney

   Pages 5-11

4. Preparation of “RNase-Free” DNase by Alkylation

   • Theodore Gurney Jr., Elizabeth G. Gurney

   Pages 13-20

5. The Isolation of Satellite DNA by Density Gradient Centrifugation

   • Craig A. Cooney, Harry R. Matthews

   Pages 21-29

6. The Isolation of High Molecular Weight Eukaryotic DNA

   • C. G. P. Mathew

   Pages 31-34

7. Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids

   • Theodore Gurney Jr.

   Pages 35-42

8. Agarose Gel Electrophoresis of DNA

   • Stephen A. Boffey

   Pages 43-50

9. Autoradiography of Gels Containing 32P

   • Verena D. Huebner, Harry R. Matthews

   Pages 51-53

10. Detection of Specific DNA Sequences—The Southern Transfer

    • C.G.P. Mathew

    Pages 55-66

11. The Extraction and Isolation of DNA from Gels

    • Wim Gaastra, Per Linå Jørgensen

    Pages 67-76

12. One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography

    • Theodore Gurney Jr.

    Pages 77-92

13. Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions

    • R. McGookin

    Pages 93-100

14. The Extraction of Total RNA by the Detergent and Phenol Method

    • Robert J. Slater

    Pages 101-108

15. RNA Extraction by the Proteinase K Method

    • R. McGookin

    Pages 109-112

16. RNA Extraction by the Guanidine Thiocyanate Procedure

    • R. McGookin

    Pages 113-116

17. The Purification of Poly(A)-Containing RNA by Affinity Chromatography

    • Robert J. Slater

    Pages 117-120

18. Messenger RNA Fractionation on Neutral Sucrose Gradients

    • R. McGookin

    Pages 121-124

19. The Estimation of mRNA Content by Poly(U) Hybridization

    • Robert J. Slater

    Pages 125-130

20. DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts

    • Jytte Josephsen, Wim Gaastra

    Pages 131-135

In recent years there has been a tremendous increase in our understanding of the functioning of the cell at the molecular level. This has been achieved in the main by the invention and development of new methodology, parti- larly in that area generally referred to as "genetic en- neering". While this revolution has been taking place in the field of nucleic acids research, the protein chemist has at the same time developed fresh methodology to keep pace with the requirements of present day molecular bi- ogy. Today's molecular biologist can no longer be content with being an expert in one particular area alone. He/she needs to be equally competent in the laboratory at h- dling DNA, RNA, and proteins, moving from one area to another as required by the problem he/she is trying to solve. Although many of the new techniques in molecular biology are relatively easy to master, it is often difficult for a researcher to obtain all the relevant information nec- sary for setting up and successfully applying a new te- nique. Information is of course available in the research l- erature, but this often lacks the depth of description that the new user requires. This requirement for in-depth pr- tical details has become apparent by the considerable - mand for places on our Molecular Biology Workshops held at Hatfield each summer.

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