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Plant Protoplast Fusion

  • Protocol
Book cover New Nucleic Acid Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 4))

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Abstract

It is now possible to regenerate plants from protoplasts of a wide range of species. As a result, genetic manipulation by protoplast fusion in vitro is now a realistic proposition. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations that cannot be obtained by conventional means. Thus, protoplast fusion can be used to introduce novel germplasm into breeding programs. Practical examples include the introduction of resistance to potato leaf roll virus from the wild species Solarium brevidens by fusion with dihaploid potato (Solatium tuberosum) protoplasts (1) and the production of novel hybrids between tomato (Lycopersicon esculentum) and the sexually incompatible wild species (Solarium rickii) (2).

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Rothamsted Experimental Station, Hertfordshire, UK

    Neil Fish, Keith Lindsey & Michael G. K. Jones

Authors
  1. Neil Fish
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  2. Keith Lindsey
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  3. Michael G. K. Jones
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Editor information

Editors and Affiliations

  1. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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© 1988 The Humana Press Inc.

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Fish, N., Lindsey, K., Jones, M.G.K. (1988). Plant Protoplast Fusion. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:481

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  • DOI: https://doi.org/10.1385/0-89603-127-6:481

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-127-2

  • Online ISBN: 978-1-59259-491-7

  • eBook Packages: Springer Protocols

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