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Quantitative Determination of Lignan Constituents from Schisandra chinensis by Liquid Chromatography

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Abstract

The fruits of Schisandra chinensis (Schisandraceae/Magnoliaceae) are a traditional oriental medicine possessing diverse biological activities. A simple and specific analytical method for the quantitative determination of eight lignan constituents from the methanolic extract of the fruits of Schisandra chinensis was developed. The lignan constituents present in the fruits of Schisandra chinensis were separated with an acetonitrile-water-reagent alcohol gradient at a flow rate of 1.0 mL per minute. The HPLC separation was performed on a Phenomenex Luna C18 (2) (150 × 4.6 mm I.D., particle size 5 μm) reversed phase column with detection at 215 nm. The limit of detection was in the range from 0.2 to1.5 μg mL−1. The relative standard deviation (RSD) values for the determination of lignan constituents in fruits of Schisandra chinensis were less than 2.0%. The method was successfully used to analyze different products available in the market containing Schisandra chinensis and also to study the percentage compositions of various lignans present in Schisandra chinensis procured from different regions in S. Korea.

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Authors and Affiliations

  1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences,  

    B. Avula, Y-W. Choi, P. V. Srinivas & I. A. Khan

  2. Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, MS, 38677, USA

    I. A. Khan

  3. Department of Horticulture, Miryang National University, Miryang, Korea

    Y-W. Choi

Authors
  1. B. Avula
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  2. Y-W. Choi
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  3. P. V. Srinivas
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  4. I. A. Khan
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Corresponding author

Correspondence to I. A. Khan.

Additional information

Revised: 24 January 2005 and 23 February 2005

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Avula, B., Choi, YW., Srinivas, P.V. et al. Quantitative Determination of Lignan Constituents from Schisandra chinensis by Liquid Chromatography. Chroma 61, 515–518 (2005). https://doi.org/10.1365/s10337-005-0547-2

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  • DOI: https://doi.org/10.1365/s10337-005-0547-2

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