Abstract
A sunscreen should form a stable and homogeneous film over the skin surface, which can improve its photoprotective activity and avoid adverse effects. For this purpose, the definition of the appropriate vehicle is of fundamental importance since emulsifying agents are known to directly influence the stability, sensorial properties and surface tension of sunscreens, modulating their film-forming performance. In this context, the objective of the present study was to systematically develop formulations with UVB/UVA protection and evaluate the effect of wax concentration on the rheological behaviour. A 2-level full factorial design was applied for the development of four formulations. Two categorical factors were evaluated, glyceryl stearate plus PEG-75 stearate (Wax 1) and methyl glucose sesquistearate (Wax 2). Rheological behaviour was determined in triplicate and rheograms were analysed using the Ostwald model. Rheological parameters were correlated by the Spearman rank correlation test and effects were evaluated by Pareto chart and surface response methodology (SRM). It was possible to identify the pseudoplastic and thixotropic behaviour of all formulations exhibiting a thinning effect on higher shear stress. Factorial analysis showed that both waxes significantly influenced consistency and thixotropic behaviour. The effect of Wax 2 concentration in thixotropy was positive and of higher magnitude and a synergistic effect was also observed. Spearman correlation coefficient of consistency index and apparent viscosity was significantly strong and positive. Finally, factorial analysis allowed the determination of the effects of waxes on the rheological parameters of the formulations. A quantitative relationship between wax concentration and significant responses was established, permitting the prediction of desirable rheological properties for improved sunscreen efficacy.
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References
Yaxi L, Xiaoyang H, Chunsheng Y, Yanyu P, Xinxin L, Guan J, et al. Photoprotection of cerium oxide nanoparticles against UVA radiation-induced senescence of human skin fibroblasts due to their antioxidant properties. Sci Rep. 2019;9:2595. https://doi.org/10.1038/s41598-019-39486-7.
Azizoglu GA, Tanriverdi ST, Kose FA, et al. AAPS PharmSciTech. 2017;18:2987. https://doi.org/10.1208/s12249-017-0786-1.
Shah P, He YY. Molecular regulation of UV-induced DNA repair. Photochem Photobiol. 2015;91(2):254–64. https://doi.org/10.1111/php.12406.
Gerst C. An eye on research. In: L’OREAL Research & Innovation. 2017. http://loreal-dam-videos-corp-en-cdn.brainsonic.com/corpen/20170713-180821-88aa8d93/attachedFiles/87f1ea2eb7153051ce58ed3a67740d79.pdf. Accessed 26 June 2019.
Federman DG, Kirsner RS, Concato J. Sunscreen counseling by US physicians. 2014;312(1):87–8. https://doi.org/10.1001/jama.2014.4320.
Liu H, Tuchinda P, Fishelevich R, Harberts E, Gaspari A. A. Human in vitro skin organ culture as a model system for evaluating DNA repair. Journal of dermatological science. 2014;74(3):236–41. https://doi.org/10.1016/j.jdermsci.2014.02.003.
Kockler J, Oelgemöller M, Robertson S, Glass B. Influence of titanium dioxide particle size on the photostability of the chemical UV-filters butyl methoxy dibenzoylmethane and octocrylene in a microemulsion. Cosmetics. 2014;1(2):128–39. https://doi.org/10.3390/cosmetics1020128.
Gianeti MD, Gaspar LR, Camargo Júnior FB, Maia Campos PMBG. Benefits of combinations of vitamin A, C and E derivatives in the stability of cosmetic formulations. Molecules. 2012;17(2):2219–30. https://doi.org/10.3390/molecules17022219.
Gaspar LR, Maia Campos PMBG. Photostability and efficacy studies of topical formulations containing UV-filters combination and vitamins A, C and E. Int J Pharm. 2007;343(1–2):181–9. https://doi.org/10.1016/j.ijpharm.2007.05.048.
Burnett ME, Hu JY, Wang SQ. Sunscreens: obtaining adequate photoprotection. Dermatologic therapy. Dermatol Ther. 2012;25(3):244–51. https://doi.org/10.1111/j.1529-8019.2012.01503.x.
Cozzi AC, Perugini P, Gourion-Arsiquaud S. Comparative behavior between sunscreens based on free or encapsulated UV filters in term of skin penetration, retention and photo-stability. Eur J Pharm Sci. 2018;121:309–18. https://doi.org/10.1016/j.ejps.2018.06.001.
Calixto LS, Maia Campos PMBG. Physical mechanical characterization of cosmetic formulations and correlation between instrumental measurements and sensorial properties. Int J Cosmet Sci. 2017;39(5):527–34. https://doi.org/10.1111/ics.12406.
Gaspar LR, Maia Campos PMBG. Rheological behavior and the SPF of sunscreens. Int J Pharm. 2003;250(1):35–44. https://doi.org/10.1016/S0378-5173(02)00462-3.
Nash JF, Tanner PR. Relevance of UV filter/sunscreen product photostability to human safety. Photodermatol Photoimmunol Photomed. 2014;30(2–3):88–95. https://doi.org/10.1111/phpp.12113.
Deng Y, Ediriwickrema A, Yang F, Lewis J, Girardi M, Saltzman WM. A sunblock based on bioadhesive nanoparticles. Nature materials. 2015;14(12):1278. https://doi.org/10.1038/nmat4422.
Bekker M, Webber GV, Louw NR. Relating rheological measurements to primary and secondary skin feeling when mineralbased and Fischer–Tropsch wax-based cosmetic emulsions and jellies are applied to the skin. Int J Cosmet Sci. 2013;35:354–61.
Sohn M, Hêche A, Herzog B, Imanidis G. Film thickness frequency distribution of different vehicles determines sunscreen efficacy. J Biomed Opt. 2014;19(11):115005. https://doi.org/10.1117/1.JBO.19.11.115005.
Ferrero L, Pissavini M, Doucet O. How a calculated model of sunscreen film geometry can explain in vitro and in vivo SPF variation. Photochem Photobiol Sci. 2010;9(4):540–51. https://doi.org/10.1039/b9pp00183b.
Teichmann A, Pissavini M, Ferrero L, Dehais A, Zastrow L, Richter H, et al. Investigation of the homogeneity of the distribution of sunscreen formulations on the human skin: characterization and comparison of two different methods. J Biomed Opt. 2006;11(6):064005. https://doi.org/10.1117/1.2409291.
Korn V, Surber C, Imanidis G. Skin surface topography and texture analysis of sun-exposed body sites in view of sunscreen application. Skin Pharmacol Physiol. 2016;29:291–9. https://doi.org/10.1159/000450760.
Hewitt J, Dahms GH. Rheology - its effect on physical SPFs. Soap Perfum Cosmet. 1996;69(3):23–5.
Chaudhary H, Kohli K, Amin S, Rathee P, Kumar V. Optimization and formulation design of gels of diclofenac and curcumin for transdermal drug delivery by Box-Behnken statistical design. J Pharm Sci. 2011;100(2):580–93. https://doi.org/10.1002/jps.22292.
Fangueiro JF, Andreani T, Egea MA, Garcia ML, Souto SB, Souto EB. Experimental factorial design applied to mucoadhesive lipid nanoparticles via multiple emulsion process. Colloids Surf B: Biointerfaces. 2012;100:84–9. https://doi.org/10.1016/j.colsurfb.2012.04.014.
Calixto LS, Infante VHP, Maia Campos PMBG. Design and characterization of topical formulations: correlations between instrumental and sensorial measurements. AAPS PharmSciTech. 2018;19(4):1512–9. https://doi.org/10.1208/s12249-018-0960-0.
Dahms GH. Einflub der Thixotropie auf die Lichtschutzwirkung von Sonnenschutzemulsionen. Parfuem Kosmet. 1994;75:675–9.
Dejaegher B, Heyden YV. Experimental designs and their recent advances in set-up, data interpretation, and analytical applications. J Pharm Biomed Anal. 2011;56:141–58.
Mukaka MM. A guide to appropriate use of correlation coefficient in medical research. Malawi Med J. 2012;24(3):69–71.
Vergilio MM, da Rocha Filho PA. Characterization, sensory and instrumental analysis of high-selling sunscreens and the correlation between its properties [dissertation]. Ribeirao Preto: University of Sao Paulo; 2018.
Vincendet M., Cebrian, J., Blasco L. Use of methyl glucoside derivatives in cosmetic compositions for the formation of liquid crystal structures improving moisturization and active penetration. In: The IP.com Prior Art Database. 2015. https://ip.com/IPCOM/000242206. Accessed 26 June 2019.
Vollhardt J, Schoop R, Janssen A, Mendrok-Edinger C, Klock J, Baltussen M, et al. The future of sunscreens: what lies beyond SPF 50+. Sofw J. 2015;141:42–8.
Al-Malah KI, Azzam MOJ, Abu-Jdayil B. Effect of glucose concentration on the rheological properties of wheat-starch dispersions. Food Hydrocolloids. 2000;14(5):491–6. https://doi.org/10.1016/S0268-005X(00)00029-1.
Cui W, Mazza G, Biliaderis CG. Chemical structure, molecular size distributions, and rheological properties of flaxseed gum. J Agric Food Chem. 1994;42(9):1891–5. https://doi.org/10.1021/jf00045a012.
Pader M. Dentifrice rheology. In: Laba D, editor. Rheological properties of cosmetic and toiletries. Marcel Dekker: New York; 1993. p. 247–73.
Gilbert L, Picard C, Savary G, Grisel M. Rheological and textural characterization of cosmetic emulsions containing natural and synthetic polymers: relationships between both data. Colloids Surf A Physicochem Eng Asp. 2013;421:150–63.
Strzelczyk AB, Jach A, Kolodziejska J, Kołodziejczyk M, Godlewska M, Piechota-Urbanska M. The use of the Gelot emulsifier in diclofenac sodium semi-solid preparations. Current Issues in Pharmacy and Medical Sciences. 2017;30(1):43–9. https://doi.org/10.1515/cipms-2017-0010.
Lee CH, Moturi V, Lee Y. Thixotropic property in pharmaceutical formulations. J Control Release. 2009;136:88–98.
Infante VHP, Calixto LS, Maia Campos PMBG. Cosmetics consumption behaviour among men and women and the importance in products indication and treatment adherence. Surg Cosmet Dermatol. 2016;8(2):134–41. https://doi.org/10.5935/scd1984-8773.201682817.
Osterwalder U, Sohn M, Herzog B. Global state of sunscreens. Photodermatol Photo. 2014;25(2–3):62–80. https://doi.org/10.1111/phpp.12112.
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This work was financially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP (grant number 2018/16523-6) and Capes (financial code 001).
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Ferreira, V.T.P., Infante, V.H.P., Felippim, E.C. et al. Application of Factorial Design and Rheology to the Development of Photoprotective Formulations. AAPS PharmSciTech 21, 46 (2020). https://doi.org/10.1208/s12249-019-1569-7
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DOI: https://doi.org/10.1208/s12249-019-1569-7