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Optimization of Curcuma Oil/Quinine-Loaded Nanocapsules for Malaria Treatment

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Abstract

Quinine, a treatment used in chloroquine-resistant falciparum malaria, was loaded into poly(ɛ-caprolactone) or Eudragit® RS100 nanocapsules using Curcuma oil as the oil-based core. Until now, the effect of cationic nanocapsules on malaria has not been reported. A 24 factorial design was adopted using, as independent variables, the concentration of Curcuma oil, presence of quinine, type of polymer, and aqueous surfactant. Diameter, zeta potential, and pH were the responses studied. The formulations were also evaluated for drug content, encapsulation efficiency, photostability, and antimalarial activity against Plasmodium berghei-infected mice. The type of polymer influenced all of the responses studied. Quinine-loaded Eudragit® RS100 (F13) and PCL nanocapsules (F9), both with polysorbate 80 coating, showed nanometric particle size, positive zeta potential, neutral pH, high drug content, and quinine photoprotection ability; thus, these nanocapsules were selected for in vivo tests. Both formulations showed lower levels of parasitemia from the beginning of the experiment (5.78 ± 3.60 and 4.76 ± 3.46% for F9 and F13, respectively) and highest survival mean time (15.3 ± 2.0 and 14.9 ± 5.6 days for F9 and F13, respectively). F9 and F13 showed significant survival curve compared to saline, thus demonstrating that nanoencapsulation improved bioefficacy of QN and co-encapsulated curcuminoids, regardless of the surface charge.

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Acknowledgements

The authors thank CNPq (Brazilian Federal Agency for the Support and Evaluation of Graduate Education) and FAPERGS (Research Support Foundation of Rio Grande do Sul) for their financial support.

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Authors and Affiliations

  1. Curso de Farmácia, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, RS, 97500-970, Brazil

    Graziela Scheuer Gomes, Eduarda Martini Piegas, Letícia Marques Colomé, Rodrigo José Freddo & Sandra Elisa Haas

  2. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, RS, 97500-970, Brazil

    Tamara Ramos Maciel, Luana Roberta Michels, Letícia Marques Colomé & Sandra Elisa Haas

  3. Programa de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, RS, 97500-970, Brazil

    Daiana Silva de Ávila

  4. Campus Bagé, Universidade Federal do Pampa, UNIPAMPA, Travessa 45, 1650, Bagé, RS, 96413-170, Brazil

    Andre Gundel

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  1. Graziela Scheuer Gomes
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  2. Tamara Ramos Maciel
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  3. Eduarda Martini Piegas
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  6. Rodrigo José Freddo
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  7. Daiana Silva de Ávila
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  8. Andre Gundel
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  9. Sandra Elisa Haas
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Correspondence to Sandra Elisa Haas.

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All animal procedures were reviewed and approved by the Animal Experimentation Ethics Committee of the Federal University of Pampa (036/2014).

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The authors declare that they have no conflict of interest.

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Gomes, G.S., Maciel, T.R., Piegas, E. et al. Optimization of Curcuma Oil/Quinine-Loaded Nanocapsules for Malaria Treatment. AAPS PharmSciTech 19, 551–564 (2018). https://doi.org/10.1208/s12249-017-0854-6

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  • DOI: https://doi.org/10.1208/s12249-017-0854-6

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