References
Li Z, Li L, Liu Y, Zhang H, Li X, Luo F, et al. Development of interferon alpha-2 microspheres with constant release. Int J Pharm. 2011;410:48–53.
Putney SD, Burke PA. Improving protein therapeutics with sustained-release formulations. Nature Biotechnol. 1998;16:153–7.
Schwendeman SP, Cardamone M, Klibanov A, Langer R. Stability of proteins and their delivery from biodegradable polymer microspheres. In: S. Cohenand, H. Bernstein (eds.) Microparticulate systems for the delivery of proteins and vaccines, Marcel Dekker, New York; 1996, p. 1–49.
Pérez C, Castellanos IJ, Constantino HR, Al-Azzam W, Griebenow K. Recent trends in stabilizing protein structure upon encapsulation and release from bioerodible polymers. J Pharm Pharmacol. 2002;54(3):301–13.
Maa YF, Hsu CC. Effect of primary emulsions on microsphere size and protein-loading in the double emulsion process. J Microencapsul. 1997;14(2):225–41.
Leach WT, Simpson DT, Val TN, Anuta EC, Yu Z, Williams 3rd RO, Johnston KP. Uniform encapsulation of stable nanoparticles produced by spray freezing for the reduction of burst release. J Pharm Sci. 2005;94(1):56–69.
Bilati U, Alléman E, Doekler E. Nanoprecipitation versus emulsion-based techniques for the encapsulation of proteins into biodegradable nanoparticles and process-related stability issues. AAPS PharmSciTech. 2005;6(4):E594–603.
Giteau A, Venier-Juliene MC, Marchal S, Courthaudon JL, Sergent M, Monteno-Menei C, et al. Reversible protein precipitation to ensure stability during encapsulation within PLGA microspheres. Eur J Pharm Biopharm. 2008;70:127–36.
Montalvo BL, Pacheco Y, Sosa BA, Vélez D, Sánchez G, Griebenow K. Formation of spherical protein nanoparticles without impacting protein integrity. Nanotechnology. 2008;19:465103.
Moharaj VJ, Chen Y. Nanoparticles- A Review. Trop J Pharm Res. 2006;5:561–73.
Al-Azzam W, Pastrana EA, King B, Mendez J, Griebenow K. Effect of the covalent modification of horseradish peroxidase with poly(ethylene glycol) on the activity and stability upon encapsulation in polyester microspheres. J Pharm Sci. 2005;94(8):1808–19.
Torchilin V. Intracellular delivery of protein and peptide therapeutics. Drug Discov Today Tech. 2008;5(2–3):e95–e103.
Soppimath KS, Aminabhavi TM. Ethyl acetate as a dispersing solvent in the production of poly(dl-lactide-co-glycolide) microspheres: effect of process parameters and polymer type. J Microencapsulation. 2002;19(3):281–92.
Castellanos IJ, Carrasquillo KG, López JD, Griebenow K. Encapsulation of bovine serum albumin in poly(lactide-co-glycolide) microspheres by the solid-in-oil-in-water technique. J Pharm Pharmacol. 2001;53(2):167–78.
Panyam J, Dali MM, Sahoo SK, Ma W, Chakravarthi SS, Amidon GL, Levy RJ, Labhasetwar V. Polymer degradation and in vitro release of a model protein from poly(d, l-lactide-co-glycolide) nano- and microparticles. J Control Release. 2003;92:173–87.
Singh R, Singh S, Lillard Jr JW. Past, present, and future technologies for oral delivery of therapeutic proteins. J Pharm Sci. 2008;97(7):2497–523.
Costantino HR, Johnson OL, Zale. Relationship between encapsulated drug particle size and initial release of recombinant human growth hormone from biodegradable microspheres. J Pharm Sci 2004; 10:2624–2634.
Yang YY, Chung TS, Ng NP. Morphology, drug distribution, and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterials. 2001;22(3):231–41.
Estey T, Kang J, Schwendenman SP, Carpenter JF. BSA degradation under acidic conditions: a model for protein instability during release from PLGA delivery systems. J Pharm Sci. 2006;95(7):1626–39.
Acknowledgments
This publication was made possible by grant number SC1 GM086240 from the National Institute for General Medical Sciences (NIGMS) at the National Institutes of Health (NIH) through the Support of Competitive Research (SCoRE) Program. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIGMS. BLM was supported by a fellowship from the NIH Research Initiative for Scientific Enhancement (RISE) Program (R25 GM061151) and by the Fellowship Program of the Puerto Rico Development Company (PRIDCO). BS was supported by a NIH-MARC fellowship (T34 GM061151).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(DOC 143 kb)
Rights and permissions
About this article
Cite this article
Montalvo-Ortiz, B.L., Sosa, B. & Griebenow, K. Improved Enzyme Activity and Stability in Polymer Microspheres by Encapsulation of Protein Nanospheres. AAPS PharmSciTech 13, 632–636 (2012). https://doi.org/10.1208/s12249-012-9794-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1208/s12249-012-9794-3