Skip to main content
Log in

Chitosan–Sodium Lauryl Sulfate Nanoparticles as a Carrier System for the In Vivo Delivery of Oral Insulin

AAPS PharmSciTech Aims and scope Submit manuscript

Abstract

The present work explores the possibility of formulating an oral insulin delivery system using nanoparticulate complexes made from the interaction between biodegradable, natural polymer called chitosan and anionic surfactant called sodium lauryl sulfate (SLS). The interaction between chitosan and SLS was confirmed by Fourier transform infrared spectroscopy. The nanoparticles were prepared by simple gelation method under aqueous-based conditions. The nanoparticles were stable in simulated gastric fluids and could protect the encapsulated insulin from the GIT enzymes. Additionally, the in vivo results clearly indicated that the insulin-loaded nanoparticles could effectively reduce the blood glucose level in a diabetic rat model. However, additional formulation modifications are required to improve insulin oral bioavailability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

REFERENCES

  1. Yamauchi K. Analysis of issues of insulin self-injection in elderly. Nippon Ronen Igakkai Zasshi (Japanese). 2009;46(6):537–40.

    Article  PubMed  Google Scholar 

  2. Rubin R, Peyrot M, Kruger D, Travis L. Barriers to insulin injection therapy: patient and health care provider perspectives. Diabetes Educ. 2009;35:1014–22.

    Article  PubMed  Google Scholar 

  3. Mastrandrea L. Inhaled insulin: overview of a novel route of insulin administration. Vasc Health Risk Manag. 2010;6:47–58.

    Article  PubMed  CAS  Google Scholar 

  4. Chien Y. Human insulin: basic sciences to therapeutic uses. Drug Dev Ind Pharm. 1996;22:753–89.

    Article  CAS  Google Scholar 

  5. Krishnankutty R, Mathew A, Sedimbi S, Suryanarayan S, Sanjeevi C. Alternative routes of insulin delivery. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009;34:933–48.

    PubMed  CAS  Google Scholar 

  6. Gomez-Perez F, Rull J. Insulin therapy: current alternatives. Arch Med Res. 2005;36:258–72.

    Article  PubMed  CAS  Google Scholar 

  7. Bailey C, Barnett A. Why is Exubera being withdrawn. BMJ. 2007;335:1156.

    Article  Google Scholar 

  8. Arbit E. The physiological rationale for oral insulin administration. Diabetes Tech Therap. 2004;6:510–7.

    Article  CAS  Google Scholar 

  9. Carino G, Mathiowitz E. Oral insulin delivery. Adv Drug Del Rev. 1999;35:249–57.

    Article  CAS  Google Scholar 

  10. Nagamoto T, Hattori Y, Takayama K, et al. Novel chitosan particles and chitosan-coated emulsions inducing immune response via intranasal vaccine delivery. Pharm Res. 2004;21:671–4.

    Article  PubMed  CAS  Google Scholar 

  11. Ohya Y, Takei T, Kobayashi H, et al. Release behaviour of 5-fluorouracil from chitosan-gel microspheres immobilizing 5-fluorouracil derivative coated with polysaccharides and their cell specific recognition. J Microencapsul. 1993;10:1–9.

    Article  PubMed  CAS  Google Scholar 

  12. Mao S, Bakowsky U, Jintapattanakit A, et al. Self-assembled polyelectrolyte nanocomplexes between chitosan derivatives and insulin. J Pharm Sci. 2006;95:1035–48.

    Article  PubMed  CAS  Google Scholar 

  13. Lavertu M, Methot S, Tran-Khanh N, et al. High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials. 2006;27:4815–24.

    Article  PubMed  CAS  Google Scholar 

  14. Sung, Hsing-wen, Lin, Yu-hsin Liang, Hsiang-faTu, Hosheng. Nanoparticles for protein drug delivery. US patent. Publication number (US 2008/0213354 A1). Publication date: 09/04/2008

  15. Sarmento B, Ribeiro AJ, Veiga F, et al. Insulin-loaded nanoparticles are prepared by alginate ionotropic pre-gelation followed by chitosan polyelectrolyte complexation. J Nanosci Nanotechnol. 2007;7:2833–41.

    Article  PubMed  CAS  Google Scholar 

  16. Ma ZT, Lim T, Lim L. Pharmacological activity of peroral chitosan–insulin nanoparticles in diabetic rats. Int J Pharm. 2005;293:271–80.

    Article  PubMed  CAS  Google Scholar 

  17. Lin YH, Mi FL, Chen CT, et al. Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery. Biomacromolecules. 2007;8:146–52.

    Article  PubMed  CAS  Google Scholar 

  18. Rekha MR, Sharma CP. Synthesis and evaluation of lauryl succinyl chitosan particles towards oral insulin delivery and absorption. J Control Release. 2009;135(2):144–15.

    Article  PubMed  CAS  Google Scholar 

  19. Qandil M, Obaidat A, Ali M, Al-taani B, Tashtoush B, Al-Jbour N, et al. Investigation of the Interactions in Complexes of Low Molecular Weight Chitosan with Ibuprofen. J Sol Chem. 2009;38:695–712.

    Article  CAS  Google Scholar 

  20. Badwan A., Al-Remawi M, Eltaher T, Elsayed A. Oral delivery of protein drug using microemulsion. International patent (WO2007/068311) date of publication 21 June 2007.

  21. Elsayed A, Remawi MA, Qinna N, Farouk A, Badwan A. Formulation and characterization of an oily-based system for oral delivery of insulin. Eur J Pharm Biopharm. 2009;73:269–79.

    Article  PubMed  CAS  Google Scholar 

  22. Edkidik N, Remawi M, Qinna N, Elsayed A, Farouk A, Badwan A. Enhancement of oral bioavailability of insulin in humans. Neuroendocrinol Lett. 2009;30:101–5.

    Google Scholar 

  23. Xu X, Fu Y, Hu H, et al. Quantitative determination of insulin entrapment efficiency in triblock copolymeric nanoparticles by high-performance liquid chromatography. J Pharm Biomed Anal. 2006;41:266–73.

    Article  PubMed  CAS  Google Scholar 

  24. Sadeghi A, Dorkoosh FM, Avadi M, et al. Preparation, characterization and antibacterial activities of chitosan, Ntrimethyl chitosan (TMC) and N-diethylmethyl chitosan (DEMC)nanoparticles loaded with insulin using both the ionotropic gelation and polyelectrolyte complexation methods. Int J Pharm. 2008;355:299–306.

    Article  PubMed  CAS  Google Scholar 

  25. El Gibaly I, Meki AM, Abdel-Ghaffar SK. Novel B melatonin-loaded chitosan microcapsules: in vitro characterization and antiapoptosis efficacy for aflatoxin B1-induced apoptosis in rat liver. Int J Pharm. 2003;260:5–22.

    Article  PubMed  Google Scholar 

  26. Berthold A, Cremer K, Kreuter J. Preparation and characterization of chitosan microspheres as drug carrier for prednisolone sodium phosphate as model for anti-inflammatory drugs. J Control Release. 1996;39:17–25.

    Article  CAS  Google Scholar 

  27. Jiang HL, Park IK, Shin NR, et al. Controlled release of Bordetella bronchiseptica dermonecrotoxin (BBD) vaccine from BBD-loaded chitosan microspheres in vitro. Arch Pharm Res. 2004;27:346–50.

    Article  PubMed  CAS  Google Scholar 

  28. Sajeesh S, Sharma CP. Cyclodextrin-insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery. Int J Pharm. 2006;325:147–54.

    Article  PubMed  CAS  Google Scholar 

  29. Zhu S, Qian F, Zhang Y, et al. Synthesis and characterization of PEG modified N-trimethylaminoethylmethacrylate chitosan nanoparticles. Eur Polym J. 2007;43:2244–53.

    Article  CAS  Google Scholar 

  30. Janes KA, Calvo P, Alonso MJ. Polysaccharide colloidal particles as delivery systems for macromolecules. Adv Drug Deliv Rev. 2001;47:83–97.

    Article  PubMed  CAS  Google Scholar 

  31. Sakuma S, Suzuki N, Kikuchi H, et al. Oral peptide delivery using nanoparticles composed of novel graft copolymers having hydrophobic backbone and hydrophilic branches. Int J Pharm. 1997;149:93–106.

    Article  CAS  Google Scholar 

  32. Dai WG, Dong LC. Characterization of physiochemical and biological properties of an insulin/lauryl sulfate complex formed by hydrophobic ion pairing. Int J Pharm. 2007;336:58–66.

    Article  PubMed  CAS  Google Scholar 

  33. Ma Z, Yeoh H, Lim LY. Formulation pH modulates the interaction of insulin with chitosan nanoparticles. J Pharm Sci. 2002;91:1396–404.

    Article  PubMed  CAS  Google Scholar 

  34. Berger J, Reist M, Mayer JM, et al. Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. Eur J Pharm Biopharm. 2004;57:35–52.

    Article  PubMed  CAS  Google Scholar 

Download references

ACKNOWLEDGMENT

This work has been carried out and financially supported by the Jordanian Pharmaceutical Manufacturing Company, Naor-Jordan.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Adnan A. Badwan.

Additional information

Guest Editors: Michael Repka, Joseph Reo, Linda Felton, and Stephen Howard

Asim Farouk—deceased

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elsayed, A., Al-Remawi, M., Qinna, N. et al. Chitosan–Sodium Lauryl Sulfate Nanoparticles as a Carrier System for the In Vivo Delivery of Oral Insulin. AAPS PharmSciTech 12, 958–964 (2011). https://doi.org/10.1208/s12249-011-9647-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1208/s12249-011-9647-5

KEY WORDS

Navigation