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Autographic Assay for the Rapid Detection of Antioxidant Capacity of Liquid and Semi-solid Pharmaceutical Formulations Using ABTS•+ Immobilized by Gel Entrapment

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Abstract

An autographic assay suitable for the detection of antioxidant compounds in a complex matrix (liquid and semi-solid pharmaceutical formulations) or in isolated compounds was described. The pre-formed radical monocation of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) was generated by oxidation of ABTS with potassium persulfate and reduced in the presence of hydrogen-donating antioxidants. For a further comparative estimation of its applicability and sensitivity, different medicinal plant extracts, hydrogels and antioxidant compounds were dot seeded or chromatographed on silica gel (TLC) and revealed with ABTS•+ solution (System I) or ABTS•+ immobilized by gel entrapment (System II). Both systems were effective and able to detect antioxidant activity in a micromolar range in seconds. System II was more sensitive and reproducible than System I. This micromethod is quick, inexpensive, and particularly helpful whether it works with numerous samples or on a small scale.

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Acknowledgments

This research was supported by grants from Consejo de Investigación de la Universidad Nacional de Tucumán, Argentina (CIUNT), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT).

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Authors and Affiliations

  1. INQUINOA. CONICET. Facultad de Bioquímica, Química y Farmacia. Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, (4000) San Miguel de Tucumán, Tucumán, Argentina

    Iris Catiana Zampini, Roxana Mabel Ordoñez & María Inés Isla

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  1. Iris Catiana Zampini
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  2. Roxana Mabel Ordoñez
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  3. María Inés Isla
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Correspondence to María Inés Isla.

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Zampini, I.C., Ordoñez, R.M. & Isla, M.I. Autographic Assay for the Rapid Detection of Antioxidant Capacity of Liquid and Semi-solid Pharmaceutical Formulations Using ABTS•+ Immobilized by Gel Entrapment. AAPS PharmSciTech 11, 1159–1163 (2010). https://doi.org/10.1208/s12249-010-9484-y

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