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MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins

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Abstract

We evaluated the evolutionary conservation of glycine myristoylation within eukaryotic sequences. Our large-scale cross-genome analyses, available as MYRbase, show that the functional spectrum of myristoylated proteins is currently largely underestimated. We give experimental evidence for in vitro myristoylation of selected predictions. Furthermore, we classify five membrane-attachment factors that occur most frequently in combination with, or even replacing, myristoyl anchors, as some protein family examples show.

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Acknowledgements

The authors are grateful for support from Boehringer Ingelheim. This project was partly funded by the Fonds zur Förderung der wissenschaftlichen Forschung Österreichs (FWF grant P15037), by the Austrian National Bank (OeNB - Österreichische Nationalbank), by the Austrian Gen-AU bioinformatics integration network (BIN) sponsored by BM-BWK, by the bioinformatics contract study 2002-2004 for BM-WA, by the Asahi Glass Foundation, Japan (to N.H.), by grants-in-aid for Scientific Research on Priority Areas 'Genome Information Science' (14015231 and 15014230) (to N.H.) and Scientific Research (C) (to N.H.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a grant-in-aid for the Fujita Health University High-tech Research Center from the Ministry of Education, Science, Sports and Culture of Japan.

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  1. IMP Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030, Vienna, Austria

    Sebastian Maurer-Stroh, Maria Novatchkova, Alexander Schleiffer, Georg Schneider, Michael Wildpaner & Frank Eisenhaber

  2. Division of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, 470-1192, Japan

    Masaki Gouda & Nobuhiro Hayashi

  3. Service de Conformation de Macromolecules Biologiques et de Bioinformatique, Université Libre de Bruxelles, Boulevard du Triomphe - CP 263, 1050, Bruxelles, Belgium

    Fernanda L Sirota

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  1. Sebastian Maurer-Stroh
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Maurer-Stroh, S., Gouda, M., Novatchkova, M. et al. MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins. Genome Biol 5, R21 (2004). https://doi.org/10.1186/gb-2004-5-3-r21

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  • DOI: https://doi.org/10.1186/gb-2004-5-3-r21

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