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Epigenetic aspects of peptidergic regulation of vascular endothelial cell proliferation in aging

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Abstract

Short peptides, vesugen and D-7, stimulated the synthesis of the proliferation-associated protein Ki-67, the expression of which decreased during aging in tissue-specific cell cultures obtained from young and old animals and in dissociated vascular endothelial cell cultures. Molecular docking methods were used to reveal the possibility of binding the vesugen and D-7 peptides to the promoter regions of the MKI67 gene, which encodes the Ki-67 protein. Both peptides have contact through the CATC sequence with core promoter 5'-agcctcaaccatcaggaaaacaagagt-3', which is located in the MKI67 gene (ENSG00000148773) from -14 to +12 bp relative to the transcriptional initiation site. Thus, the vasoprotective effect of vesugen, which was previously revealed in elder people, can be manifested through epigenetic regulation of the Ki-67 gene expression.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, nab. Makarova 6, St. Petersburg, 199034, Russia

    V. Kh. Khavinson

  2. St. Petersburg Institute of Bioregulation and Gerontology, pr. Dinamo 3, St. Petersburg, 197110, Russia

    V. Kh. Khavinson, S. I. Tarnovskaya, N. S. Linkova, E. O. Gutop & E. V. Elashkina

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  1. V. Kh. Khavinson
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  2. S. I. Tarnovskaya
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  3. N. S. Linkova
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  4. E. O. Gutop
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  5. E. V. Elashkina
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Correspondence to V. Kh. Khavinson.

Additional information

Original Russian Text © V.Kh. Khavinson, S.I. Tarnovskaya, N.S. Linkova, E.O. Gutop, E.V. Elashkina, 2014, published in Uspekhi Gerontologii, 2014, Vol. 27, No. 1, pp. 108–114.

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Khavinson, V.K., Tarnovskaya, S.I., Linkova, N.S. et al. Epigenetic aspects of peptidergic regulation of vascular endothelial cell proliferation in aging. Adv Gerontol 5, 219–224 (2015). https://doi.org/10.1134/S2079057015040116

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