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Staphylococcal enterotoxin a detection with phage displayed antibodies

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Abstract

A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6–12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.

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Abbreviations

BSA:

bovine serum albumin

PBS:

phosphate buffered saline

PBST:

PBS with 0.1% Tween 20

PBST-M:

PBST with 2% milk powder

scFv:

single chain variable fragment mini-antibody

SEA and SEE:

Staphylococcal enterotoxins A and E

ELISA:

enzyme immunoassay

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Authors and Affiliations

  1. Ernst Institute of Animal Husbandry, Dubrovitsy settlement, Podolsk District, Moscow oblast, 142132, Russia

    K. K. Fursova, M. P. Shchannikova, A. O. Shepelyakovskaya & F. A. Brovko

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch), Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    K. K. Fursova, M. P. Shchannikova, A. O. Shepelyakovskaya & F. A. Brovko

  3. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    L. L. Pavlik

Authors
  1. K. K. Fursova
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  2. M. P. Shchannikova
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  3. A. O. Shepelyakovskaya
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  4. L. L. Pavlik
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  5. F. A. Brovko
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Correspondence to F. A. Brovko.

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Original Russian Text © K.K. Fursova, M.P. Shchannikova, A.O. Shepelyakovskaya, L.L. Pavlik, F.A. Brovko, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 3, pp. 327–333.

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Fursova, K.K., Shchannikova, M.P., Shepelyakovskaya, A.O. et al. Staphylococcal enterotoxin a detection with phage displayed antibodies. Russ J Bioorg Chem 42, 294–299 (2016). https://doi.org/10.1134/S1068162016020035

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  • DOI: https://doi.org/10.1134/S1068162016020035

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