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Investigation of Erythrocyte Transport through Microchannels After the Induction of Oxidative Stress with Tert-Butyl Peroxide

  • NANODIAGNOSTICS AND PROBE TECHNOLOGIES IN BIOLOGY AND MEDICINE
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Abstract

To characterize the effect of oxidative stress (OS) on the ability of erythrocytes to pass through microvessels and capillaries, we studied the dynamics of the movement of human erythrocytes in vitro, in the channels of a microfluidic device, under the action of an OS inducer, tert-butyl hydroperoxide (tBH), and compared it with a cytological assessment of the transformation of cell membranes. OS induced the impairment of the control over the shape and volume of cells, the decrease in passage speed, and the occlusions of microchannels. The developed microfluidic device provided an assessment of the microreology of erythrocytes under the action of hydroperoxide. In the future, it is possible to use microfluidic analysis of erythrocytes in patients to assess the effect of xenobiotics on microrheology.

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. 20-34-70111 “Stability.”

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Authors and Affiliations

  1. Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 194223, St. Petersburg, Russia

    E. A. Skverchinskaya & I. V. Mindukshev

  2. St. Petersburg National Research Academic University, 194021, St. Petersburg, Russia

    O. D. Tapinova, N. A. Filatov, N. A. Besedina & A. S. Bukatin

  3. Institute for Analytical Instrumentation, Russian Academy of Sciences, 190103, St. Petersburg, Russia

    A. S. Bukatin

Authors
  1. E. A. Skverchinskaya
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  2. O. D. Tapinova
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  3. N. A. Filatov
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  4. N. A. Besedina
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  5. I. V. Mindukshev
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  6. A. S. Bukatin
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Corresponding author

Correspondence to A. S. Bukatin.

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COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed in a study of human beings comply with ethical standards. Blood samples were obtained from healthy volunteers after obtaining written consent. The study followed the principles of the Helsinki Declaration (according to the 64th WMA General Assembly, Brazil, 2013) and was approved by the Ethics Committee of the Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences (protocol no. 15 of November 21, 2017).

CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

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Translated by I. Shipounova

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Skverchinskaya, E.A., Tapinova, O.D., Filatov, N.A. et al. Investigation of Erythrocyte Transport through Microchannels After the Induction of Oxidative Stress with Tert-Butyl Peroxide. Tech. Phys. 65, 1491–1496 (2020). https://doi.org/10.1134/S1063784220090236

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  • DOI: https://doi.org/10.1134/S1063784220090236

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