Abstract
An inorganic film of ruthenium hexachlororuthenate (RuRuCl6), deposited on the surface of a glassy carbon electrode, exhibits electrocatalytic activity in the oxidation of purine nucleosides, such as guanosine and adenosine. Appropriate operating conditions are found for fabricating a polymer film on the surface of glassy carbon and for recording the maximum electrocatalytic current for the modified electrode. A method for the selective voltammetric determination of guanosine and adenosine in their simultaneous presence at an electrode modified by a RuRuCl6 film is developed. A procedure is proposed for the amperometric detection of purine nucleosides with this modified electrode under the conditions of flow-injection analysis. The linear dependence of the analytical signal on the concentration of guanosine and adenosine is observed up to 5 × 10–6 M in the stationary mode and up to 5 × 10–7 M in the flow system. The proposed method for the selective determination of guanosine and adenosine was tested in the analysis of human urine.
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Original Russian Text © L.G. Shaidarova, A.V. Gedmina, V.D. Demina, I.A. Chelnokova, H.C. Budnikov, 2018, published in Zhurnal Analiticheskoi Khimii, 2018, Vol. 73, No. 4, pp. 298–307.
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Shaidarova, L.G., Gedmina, A.V., Demina, V.D. et al. Selective Voltammetric and Flow-Injection Determination of Guanosine and Adenosine at a Glassy Carbon Electrode Modified with a Ruthenium Hexachlororuthenate Film. J Anal Chem 73, 390–398 (2018). https://doi.org/10.1134/S1061934818040111
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DOI: https://doi.org/10.1134/S1061934818040111