Skip to main content
SpringerLink
Log in

Determination of growth hormones (β-agonists) in muscle tissue by HPLC with mass spectrometric detection

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

A procedure is developed for determining 11 β-agonists, that is, hormonal growth promoters for farm animals, using HPLC with mass spectrometric detection. The procedure includes the enzymatic hydrolysis of homogenates of organs and tissues of animals (longissimus muscle, liver, and kidney), precipitation of proteins with perchloric acid, extraction of β-agonists with a mixture of isopropyl alcohol–ethyl acetate (6: 4), purification of the extract by solid phase extraction using a Bond Elut cartridge, HPLC separation in the gradient elution mode, electrospray ionization, and mass spectrometric detection in the multiple reaction monitoring mode. The procedure is certified. The analytical range is 1.0–100.0 μg/kg.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Scientific opinion of the panel on additives and products or substances used in animal feed (FEEDAP) on a request from the European Commission on the safety evaluation of ractopamine, EFSA J., 2009, vol. 1041, p. 52.

    Google Scholar 

  2. El-Armouche, A., Gocht, F., and Jaeckel, E., Eur. J. Heart Failure, 2007, vol. 9, p. 1077.

    Article  CAS  Google Scholar 

  3. Vidal, M., Wieland, T., Lohse, M.J., and Lorenz, K., Cardiovasc. Res., 2012, vol. 96, p. 255.

    Article  CAS  Google Scholar 

  4. Pearen, M.A., Ryall, J.G., Lynch, G.S., and Muscat, G., BMC Genomics, 2009, vol. 10, p. 448.

    Article  Google Scholar 

  5. Council Directive 96/22/ES of 29 April 1996 concerning on the use in stockfarming of certain substances having a hormonal or thyrostatic action and of betaagonists, and repealing Directives 81/602/EEC, 88/146/EEC and 88/299/EE., Off. J. Eur. Comm., 1996, vol. 125, p. 3.

    Google Scholar 

  6. Eurasian Economic Commission. Uniform sanitary and epidemiological and hygienic requirements for goods subject to sanitary-and-epidemiologic supervision (control), approved May 28, 2010, decision no. 299. http://www.tsouz.ru/kts/kts17/pages/p2_299.aspx.

  7. Anonymous (1996) Council Directive 96/23/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products, and repealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC, and in particular Art. 2., Off. J. Eur. Comm., 1996, vol. 125, p. 10.

    Google Scholar 

  8. GOST (State Standard) R 54032-2010: Food Products, Feed, Food Raw Materials. Method of Determination of ß-Agonists by Gas Chromatography with Mass Spectrometric Detection, Moscow: Standartinform, 2011.

  9. Bing Sha., Xiaofei Jia, Jing Zhang, Juan Meng, Yongning Wu, Hejun Duan, and Xiaoming Tu, Food Chem., 2009, vol. 114, no. 3, p. 1115.

    Article  Google Scholar 

  10. Fan Sa., Miao Hong, Zhao Yunfeng, Chen Huijing, and Wu Yongning, J. Agric. Food Chem., 2012, vol. 60, no. 8, p. 1898.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Volga Research Institute of Production and Processing of Meat and Dairy Products, ul. im. Marshala Rokossovskogo 6, Volgograd, 400131, Russia

    A. V. Kulikovskii, I. F. Gorlov & M. I. Slozhenkina

  2. Gorbatov All-Russia Research Institute of Meat Industry, ul. Talalikhina 26, Moscow, 109316, Russia

    A. B. Lisitsyn

  3. Sechenov First Moscow State Medical University, Trubetskaya ul. 8 build. 2, Moscow, 119991, Russia

    S. A. Savchuk

Authors
  1. A. V. Kulikovskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. B. Lisitsyn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. F. Gorlov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. I. Slozhenkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. A. Savchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Kulikovskii.

Additional information

Original Russian Text © A.V. Kulikovskii, A.B. Lisitsyn, I.F. Gorlov, M.I. Slozhenkina, S.A. Savchuk, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 10, pp. 1101–1105.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kulikovskii, A.V., Lisitsyn, A.B., Gorlov, I.F. et al. Determination of growth hormones (β-agonists) in muscle tissue by HPLC with mass spectrometric detection. J Anal Chem 71, 1052–1056 (2016). https://doi.org/10.1134/S1061934816100075

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934816100075

Keywords

Search

Navigation