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Applicability of the critical chromatography concept to proteomics problems: I. Effect of the stationary phase and the size of the chromatographic column on the dependence of the retention time of peptides and proteins on the amino acid sequence

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Abstract

A theoretical study of the effect of stationary phase parameters on the regularities of the separation of peptides and proteins using a model of critical chromatography BioLCCC and the Theoretical chromatography software created on its basis has been performed. The following problems have been discussed: mechanism of the migration of chromatographic peak along the column and its compression in gradient elution; dependence of the coefficient of protein separation on the column length; inversion of the elution order of peptides at a change of the diameter or length of the column, adsorbent nature, conditions of emergence of inversion; and anomaly of the dependence of the retention time of proteins on the adsorbent activity.

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Authors and Affiliations

  1. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia

    A. V. Gorshkov & V. V. Evreinov

  2. Tal’roze Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38, build. 2, Moscow, 119334, Russia

    M. L. Pridatchenko, T. Yu. Perlova, I. A. Tarasova & M. V. Gorshkov

Authors
  1. A. V. Gorshkov
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  2. M. L. Pridatchenko
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  3. T. Yu. Perlova
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  4. I. A. Tarasova
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  5. M. V. Gorshkov
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  6. V. V. Evreinov
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Corresponding author

Correspondence to V. V. Evreinov.

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Original Russian Text © A.V. Gorshkov, M.L. Pridatchenko, T.Yu. Perlova, I.A. Tarasova, M.V. Gorshkov, V.V. Evreinov, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 1, pp. 113–128.

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Gorshkov, A.V., Pridatchenko, M.L., Perlova, T.Y. et al. Applicability of the critical chromatography concept to proteomics problems: I. Effect of the stationary phase and the size of the chromatographic column on the dependence of the retention time of peptides and proteins on the amino acid sequence. J Anal Chem 71, 110–125 (2016). https://doi.org/10.1134/S1061934816010056

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  • DOI: https://doi.org/10.1134/S1061934816010056

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