Abstract
Fluorescence spectrometry is used to study the interaction between hydrazone formed by pyridoxal-5-phosphate and 4-hydroxybenzoic acid hydrazide with bovine serum albumin in solution. The constant of bonding hydrazone to the protein is calculated in two ways, and the calculation procedures are discussed. A solution containing hydrazone, La3+ ions, and bovine serum albumin is studied spectrophotometrically. It is shown that a rise in the concentration of protein gradually destroys the lanthanum(III)–hydrazone complex, due to the ligand bonding to the protein.
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ACKNOWLEDGMENTS
This work was performed on equipment at the Ivanovo State University of Chemistry and Technology’s shared resource center with the support of the RF Ministry of Science and Higher Education, agreement no. 075-15-2021-671.
Funding
The theoretical part of this work was performed, and our spectrophotometric measurements were made, at the Ivanovo State University of Chemistry and Technology’s Research Institute of Thermodynamics and Kinetics of Chemical Processes, as part of a State Assignment from the RF Ministry of Science and Higher Education, project FZZW-2020-0009. Our spectrofluorimetric experiments were supported by the RF Presidential Grant Council, project SP-1556.2021.4.
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Translated by G. Levit
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Materials from this work were presented at the XIV International Scientific Conference held in Ivanovo from September 20 to 24, 2021.
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Yarullin, D.N., Zavalishin, M.N., Sharnin, V.A. et al. Equilibrium in a Bovine Serum Albumin–Pyridoxal-5-phosphate 4-Hydroxybenzoyl Hydrazone–La3+ Ion System. Russ. J. Phys. Chem. 96, 1190–1194 (2022). https://doi.org/10.1134/S0036024422060310
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DOI: https://doi.org/10.1134/S0036024422060310