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ZNF143 is involved in CTCF-mediated chromatin interactions by cooperation with cohesin and other partners

  • Molecular Cell Biology
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Abstract

ZNF143 is a ubiquitously expressed transcription factor conserved in vertebrates and might regulate the expression of numerous genes. But its function in mediating chromatin interactions remains elusive. By integrated analysis of public datasets, we provided evidence that a majority of ZNF143 binding sites (BSs) were involved in CTCF-mediated chromatin interaction networks (CTCF-CINs) by overlapping with cohesin-BSs and CTCF-BSs. We further showed that only a very few CTCF-CINs were associated with ZNF143 alone, whereas those associated with ZNF143 and cohesin simultaneously were highly overlapped with constitutive, conserved CTCF-BSs and enriched at boundaries of chromatin topologically associating domains. These observations implicate that as an important partner of CTCF, ZNF143 helps it establish the conserved chromatin structure by cooperating with cohesin.

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Abbreviations

BS:

binding site

CIN:

chromatin interaction network

TAD:

topologically associating domain

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Authors and Affiliations

  1. College of Life Sciences, Capital Normal University, 105 Xisanhuanbei Road, Haidian District, Beijing, 100048, China

    B.-Y. Ye & F.-X. Zhang

  2. Beijing Institute of Biotechnology, No. 20, Dongdajie Street, Fengtai District, Beijing, 100071, P. R. China

    B.-Y. Ye, W.-L. Shen, D. Wang, P. Li, Z. Zhang, M.-L. Shi, Y. Zhang & Z.-H. Zhao

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  1. B.-Y. Ye
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  2. W.-L. Shen
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  3. D. Wang
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  4. P. Li
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  5. Z. Zhang
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  6. M.-L. Shi
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  7. Y. Zhang
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  8. F.-X. Zhang
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  9. Z.-H. Zhao
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Correspondence to F.-X. Zhang or Z.-H. Zhao.

Additional information

Published in Russian in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 3, pp. 496–503.

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Ye, BY., Shen, WL., Wang, D. et al. ZNF143 is involved in CTCF-mediated chromatin interactions by cooperation with cohesin and other partners. Mol Biol 50, 431–437 (2016). https://doi.org/10.1134/S0026893316030031

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  • DOI: https://doi.org/10.1134/S0026893316030031

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