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Bacterial Adhesion and Biofilm Formation in the Presence of Chitosan and Its Derivatives

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Abstract—

The effect of chitosan and its derivatives on adhesion and biofilm formation by bacteria with diametrically opposite properties of cell surfaces was studied. Treatment of polystyrene surface with chitosan or its quaternized derivatives was shown to decrease its hydrophobicity significantly, resulting with an over fourfold inhibition of M. smegmatis adhesion. On the contrary, E. coli with hydrophilic cell surface showed higher affinity to the polystyrene surface in the presence of chitosan. The possibility of inhibiting formation of M. smegmatis biofilms by chitosan, which increases its antimycobacterial properties after its quaternization, has not been demonstrated previously. At the same time, the increase in the hydrophobicity of quaternized chitosan resulted in its decreased efficiency against E. coli cells with a relatively hydrophilic surface. The succinylated form of chitosan with negatively charged sites in the structure had practically no antibacterial properties due its decreased ability to bind with the target cells.

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ACKNOWLEDGMENTS

This study was carried out as part of the state task (state registration no. 01201353249) and supported by the Comprehensive Research Program of the Russian Academy of Sciences, Ural Branch (project no. 18-7-8-8).

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia

    T. V. Polyudova & V. P. Korobov

  2. Perm State Agro-Technological University, 614990, Perm, Russia

    T. V. Polyudova

  3. Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    B. Ts. Shagdarova & V. P. Varlamov

  4. Perm National Research Polytechnic University, 614990, Perm, Russia

    V. P. Korobov

Authors
  1. T. V. Polyudova
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  2. B. Ts. Shagdarova
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  3. V. P. Korobov
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  4. V. P. Varlamov
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Correspondence to T. V. Polyudova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Panyushkina

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Polyudova, T.V., Shagdarova, B.T., Korobov, V.P. et al. Bacterial Adhesion and Biofilm Formation in the Presence of Chitosan and Its Derivatives. Microbiology 88, 125–131 (2019). https://doi.org/10.1134/S0026261719020085

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  • DOI: https://doi.org/10.1134/S0026261719020085

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