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Candidatus ‘Chloroploca asiatica’ gen. nov., sp. nov., a new mesophilic filamentous anoxygenic phototrophic bacterium

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Abstract

Five phylogenetically similar monocultures of mesophilic filamentous anoxygenic phototrophic bacteria (FAPB) were isolated from microbial mats of low-mineral (5–28 g/L) alkaline lakes in Buryat Republic, Transbaikalia and Mongolia, as well as from biofilms of an alkaline sulfide spring (3 g/L) of the Umhei hydrothermal system (Buryat Republic). New isolates were characterized by short trichomes (15–30 μm long and ∼1 μm in diameter), straight, curved, or wavy, surrounded by a thin iron-sorbing mucous sheath. Gliding motion of the trichomes was not observed. The trichomes formed bunches consisting of several filaments. Trichomes multiply by the separation of short fragments or single cells from the parental trichome. The cells in the filaments were elongated; they contained chlorosomes, gas vesicles, poly-β-hydroxybutyrate granules, and small polyphosphate inclusions. Bacteria contained bacteriochlorophylls c and a and γ-carotene. Absorption maxima of the pigments in the cells were observed at 462, (shoulder at 515), 742, 805, and 863 nm. The organisms were strict anaerobes capable of photoautotrophic growth with sulfide as an electron donor. Elemental sulfur emerged into the medium as a result of sulfide photooxidation. The organisms were tolerant to sulfide (up to 8 mM). Best growth occurred at pH 8.0, 3–15 g/L NaCl, and 1–5 g/L sodium bicarbonate. According to phylogenetic analysis, the 16S rRNA gene sequences of the FAPB isolates formed a separate cluster most closely related to the species cluster of the family Oscillochloridaceae, suborder Chloroflexinae, order Chloroflexales, class Chloroflexi. The differences with the closest 16S rRNA gene sequences of the known FAPB were 9–10%. The formal description of a new taxon, Candidatus’ Chloroploca asiatica’ gen. nov., sp. nov., is provided.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    V. M. Gorlenko, I. A. Bryantseva, A. M. Kalashnikov & V. A. Gaisin

  2. Centre “Bioengineering,” Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia

    V. A. Gaisin, M. V. Sukhacheva, D. S. Gruzdev & B. B. Kuznetsov

Authors
  1. V. M. Gorlenko
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  2. I. A. Bryantseva
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  3. A. M. Kalashnikov
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  4. V. A. Gaisin
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  5. M. V. Sukhacheva
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  6. D. S. Gruzdev
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  7. B. B. Kuznetsov
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Correspondence to V. M. Gorlenko.

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Gorlenko, V.M., Bryantseva, I.A., Kalashnikov, A.M. et al. Candidatus ‘Chloroploca asiatica’ gen. nov., sp. nov., a new mesophilic filamentous anoxygenic phototrophic bacterium. Microbiology 83, 838–848 (2014). https://doi.org/10.1134/S0026261714060083

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  • DOI: https://doi.org/10.1134/S0026261714060083

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