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Dormant state and phenotypic variability of Staphylococcus aureus and Corynebacterium pseudodiphtheriticum

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Abstract

Ability to produce dormant forms (DF) was demonstrated for non-spore-forming bacteria Staphylococcus aureus (a nonpathogenic strain) and Corynebacterium pseudodiphtheriticum (an organism of the normal oropharyngeal flora). The salient features of the sthaphylococcal and corynebacterial DF were (1) prolonged (4 months) preservation of viability; (2) resistance to damaging factors (heat treatment); and (3) specific morphology and ultrastructure. The optimal conditions for DF formation were (1) transfer of stationary-phase cultures into saline solution with CaCl2 (10–300 mM) (for S. aureus); (2) growth in SR1 synthetic medium with fivefold nitrogen limitation (for C. pseudodiphtheriticum); and (3) incubation with (1–5) × 10−4 M of C12-AHB, an alkylhydroxybenzene akin to microbial anabiosis autoinducers. Increase of C12-AHB concentration to 7 × 10−4–2 × 10−3 M resulted in “mummification” of cells with irreversible loss of viability without autolytic processes. Germination of dormant forms was followed by increasing of phenotypic variability, as seen from (1) diversity of colony types and (2) emergence of antibiotic-resistant clones on selective media. The share of kanamycin-resistant S. aureus variants was most numerous (0.002–0.01%) in 4-month DF suspensions in SALINE with CaCl2. In the C. pseudodiphtheriticum DF produced under the effect of C12-AHB, the share of kanamycin-resistant variants was also found to increase. These data point to an association between the emergence of antibiotic-resistant variants of bacteria and their persistence in dormant state mediated by starvation stress and regulated by AHB.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    A. L. Mulyukin, V. F. Gal’chenko & G. I. El’-Registan

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia

    N. E. Suzina

  3. International Science & Technology Center, Moscow, Russia

    V. G. Mel’nikov

Authors
  1. A. L. Mulyukin
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  2. N. E. Suzina
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  3. V. G. Mel’nikov
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  4. V. F. Gal’chenko
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  5. G. I. El’-Registan
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Correspondence to A. L. Mulyukin.

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Original Russian Text © A.L. Mulyukin, N.E. Suzina, V.G. Mel’nikov, V.F. Gal’chenko, G.I. El’-Registan, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 1, pp. 15–27.

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Mulyukin, A.L., Suzina, N.E., Mel’nikov, V.G. et al. Dormant state and phenotypic variability of Staphylococcus aureus and Corynebacterium pseudodiphtheriticum . Microbiology 83, 149–159 (2014). https://doi.org/10.1134/S0026261713060088

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  • DOI: https://doi.org/10.1134/S0026261713060088

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