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Method for rapid DNA extraction from bacterial communities of different soils

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Abstract

A method for indirect DNA extraction from various soils significantly differing in their physicochemical properties has been developed. The proposed method is based on cell desorption from soil particles using a Tris-EDTA (TE) buffer supplemented with polyvinylpolypyrrolydone (PVPP) and sodium dodecylsulfate (SDS). Methods for subsequent cell lysis and purification of DNA preparations based on alkaline lysis followed by chromatography on ion-exchange resins were described by us earlier. The purity of the DNA preparations obtained did not depend on the type of soil. It was shown that the DNA preparations can be used for the amplification of rather large fragments, e.g., sequences spanning the complete 16S rRNA gene.

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Authors and Affiliations

  1. Bioengineering Center, Russian Academy of Sciences, Russia

    E. V. Zaporozhenko, N. V. Slobodova, E. S. Boulygina & B. B. Kuznetsov

  2. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, Bld. 2, Moscow, 117811, Russia

    I. K. Kravchenko

Authors
  1. E. V. Zaporozhenko
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  2. N. V. Slobodova
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  3. E. S. Boulygina
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  4. I. K. Kravchenko
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  5. B. B. Kuznetsov
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Correspondence to E. S. Boulygina.

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Original Russian Text © E.V. Zaporozhenko, N.V. Slobodova, E.S. Boulygina, I. K. Kravchenko, B.B. Kuznetsov, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 1, pp. 127–134.

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Zaporozhenko, E.V., Slobodova, N.V., Boulygina, E.S. et al. Method for rapid DNA extraction from bacterial communities of different soils. Microbiology 75, 105–111 (2006). https://doi.org/10.1134/S0026261706010188

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  • DOI: https://doi.org/10.1134/S0026261706010188

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