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Conformation of Polymer-Carrier Macromolecules and the Activity of Immobilized Enzyme

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Abstract

The dependence of the activity of immobilized trypsin on the conformation of the polymer carrier macromolecule was studied. Fluorescence spectroscopy shows that immobilization of the enzyme on a rigid polymer chain prevents the association of enzyme molecules and ensures the availability of its active centers, even for high-molecular-weight molecules.

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Funding

This work was performed within the framework of governmental task of the Institute of Petrochemical Synthesis of the Russian Academy of Sciences.

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Correspondence to I. L. Valuev.

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The authors declare that they do not have conflict of interest. The study was performed without the use of animals and without the involvement of human subjects.

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Translated by P. Kuchina

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Valuev, I.L., Vanchugova, L.V. & Valuev, L.I. Conformation of Polymer-Carrier Macromolecules and the Activity of Immobilized Enzyme. Appl Biochem Microbiol 56, 29–31 (2020). https://doi.org/10.1134/S0003683820010159

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  • DOI: https://doi.org/10.1134/S0003683820010159

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