Abstract
The effect of the main characteristics of low molecular weight chitosan obtained via enzymatic and chemical hydrolysis with a molecular mass (MM) of 2–100 kDa, a deacetylation degree (DD) of 60–98%, and a polydispersity (Mw/Mn) of 1.9–2.7 on the growth of Botrytis cinerea has been studied. The greatest inhibitory effect on the fungal germination process (a conidia germination index of less than 50%) was provided by chitosan samples with an MM of 2–13 kDa, a DD of 85–98%, and a polydispersity of 2–2.5. Among the most effective was chitosan with an MM of 13 kDa and a deacetylation degree 98%, which had a significant inhibitory effect on the growth of the fungal mycelium at concentration 0.938 mg/mL. This sample of chitosan and its complex with copper ions (75 ppm) significantly suppressed fungal metabolic activity (up to 20 and 10%, respectively), which indicates their high potential as antifungal agents for the development of fungicides.
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ACKNOWLEDGMENTS
The authors are grateful to S.A. Lopatin (Laboratory of Biopolymer Engineering, Institute of Bioengineering, Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences) for the high-efficiency gel permeation chromatography of the chitosan samples.
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Karpova, N.V., Shagdarova, B.T., Lyalina, T.S. et al. Influence of the Main Characteristics of Low Weight Chitosan on the Growth of the Plant Pathogenic Fungus Botrytis сinerea. Appl Biochem Microbiol 55, 405–413 (2019). https://doi.org/10.1134/S0003683819040069
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DOI: https://doi.org/10.1134/S0003683819040069