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Discoloration of the azo dye Congo Red by manganese-dependent peroxidase from Pleurotus sajor caju

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Abstract

This paper describes the production of ligninolytic enzymes by the white-rot fungus Pleurotus sajor caju under solid-state fermentation conditions using a cost-effective medium consisting of agro-industrial wastes. From the different agro-industrial wastes tested (i.e. orange, banana, mango and cantaloupe peels), banana peels led to the highest manganese-dependent peroxidase (MnP) activity (6.3 U/mL on the 10 day). MnP from banana peel cultures was purified and applied to the discoloration of the azo dye Congo Red (CR). The optimum temperature, pH and enzyme concentration for maximum discoloration (i.e. 95% in 1 h) were found to be 35°C, 4.0, and 1.4 U/mL, respectively. In addition, the phytotoxicity (with respect to Sorghum vulgare and Phaseolus radiatus seeds) of CR was considerably reduced after the treatment of plant material with MnP produced by P. sajor caju. The products obtained after discoloration of CR were characterized using GC/MS as 8-amino naphthol 3-sulfonic acid, 3-hydroperoxy 8-nitrosonaphthol, p-p'-dihydroxybiphenyl. Therefore, this approach holds promise for the production and application of MnP from P. sajor caju on a larger scale.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, 12613, Egypt

    R. S. Yehia

  2. CEIT-IK4, Unit of Environmental Engineering, Paseo Manuel de Lardizabal 15, San Sebastian, 20018, Spain

    S. Rodriguez-Couto

  3. IKERBASQUE, Basque Foundation for Science, María Díaz de Haro 3, Bilbao, 48013, Spain

    S. Rodriguez-Couto

Authors
  1. R. S. Yehia
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Correspondence to R. S. Yehia.

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Yehia, R.S., Rodriguez-Couto, S. Discoloration of the azo dye Congo Red by manganese-dependent peroxidase from Pleurotus sajor caju . Appl Biochem Microbiol 53, 222–229 (2017). https://doi.org/10.1134/S0003683817020181

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