Abstract
Genetic constructs coding for the fusion of the Mam12 membrane protein from the magnetotactic bacteria Magnetospirilium magnetotacticum with one B-domain (mb) or two B or Z domains (mbb and mzz, respectively) of Staphylococcus aureus protein A have been synthesized. Expression, fractionation, and purification of fusion proteins have been performed, and the activity of the modified proteins has been characterized. ELISA results showed that all modified proteins displayed immunoglobulin-binding activity.
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Original Russian Text © D.S. Grouzdev, M.V. Dziuba, A.S. Gerasimov, B.B. Kuznetsov, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 3, pp. 242–248.
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Grouzdev, D.S., Dziuba, M.V., Gerasimov, A.S. et al. Production of modified magnetosome membrane proteins and analysis of their activity. Appl Biochem Microbiol 49, 220–226 (2013). https://doi.org/10.1134/S0003683813030095
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DOI: https://doi.org/10.1134/S0003683813030095