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Chemical characterization and anti-inflammatory effect of rauvolfian, a pectic polysaccharide of Rauvolfia callus

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Abstract

The pectic polysaccharide named rauvolfian RS was obtained from the dried callus of Rauvolfia serpentina L. by extraction with 0.7% aqueous ammonium oxalate. Crude rauvolfian RS was purified using membrane ultrafiltration to yield the purified rauvolfian RSP in addition to glucan as admixture from the callus, with molecular weights 300 and 100–300 kD, respectively. A peroral pretreatment of mice with the crude and purified samples of rauvolfian (RS and RSP) was found to decrease colonic macroscopic scores, the total area of damage, and tissue myelope roxidase activity in colons as compared with a colitis group. RS and RSP were shown to stimulate production of mucus by colons of the colitis mice. RSP appeared to be an active constituent of the parent RS. The glucan failed to possess anti-inflammatory activity.

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Abbreviations

LPS:

lipopolysaccharides

MPO:

myeloperoxidase

RS:

initial rauvolfian

RSH:

galacturonan

RSP:

low methyl esterified pectin

SCFAs:

short chain fatty acids

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Authors and Affiliations

  1. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia

    S. V. Popov, O. A. Patova, P. A. Markov, I. R. Nikitina, R. G. Ovodova, G. Yu. Popova & Yu. S. Ovodov

  2. Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Republic of Tatarstan, Russia

    V. G. Vinter

  3. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, 117930, Moscow, Russia

    A. S. Shashkov

Authors
  1. S. V. Popov
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  2. V. G. Vinter
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  3. O. A. Patova
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  4. P. A. Markov
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  5. I. R. Nikitina
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  6. R. G. Ovodova
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  7. G. Yu. Popova
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  8. A. S. Shashkov
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  9. Yu. S. Ovodov
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Corresponding author

Correspondence to Yu. S. Ovodov.

Additional information

Published in Russian in Biokhimiya, 2007, Vol. 72, No. 7, pp. 955–962.

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Popov, S.V., Vinter, V.G., Patova, O.A. et al. Chemical characterization and anti-inflammatory effect of rauvolfian, a pectic polysaccharide of Rauvolfia callus. Biochemistry Moscow 72, 778–784 (2007). https://doi.org/10.1134/S0006297907070139

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  • DOI: https://doi.org/10.1134/S0006297907070139

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