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2004 SIVB Congress Symposium Proceedings “Thinking Outside the Cell”: Plant Protoplast Technology: Status and Applications

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Summary

Plant protoplasts provide an enabling technology to underpin aspects of development, physiology, and genetics. Reliable procedures are available to isolate and culture protoplasts from monocotyledons and dicotyledons. Several parameters influence the topipotency of protoplasts and their derived cells, particularly the source tissue, culture medium, and environmental factors. Novel approaches to maximize the efficiency of protoplast-to-plant systems include techniques already established for animal and microbial cells, such as electrostimulation and exposure of protoplasts to surfactants and artificial respiratory gas carriers, especially perfluorochemicals and hemoglobin. Somatic hybridization by protoplast fusion is undergoing a resurgence of interest, since it enables nuclear and cytoplasmic genomes to be combined at the interspecific and intergeneric levels without prior knowledge of gene location, or involvement of recombinant DNA technology. DNA uptake into protoplasts has applications in transient and stable transformation, including the generation of transplastomic plants of commercial importance in molecular pharming. Other applications of isolated protoplasts are in studies of membrane function, cell structure, and longer-term toxicological assessments. Despite the century that has elased since protoplasts were first isolated, they still make a significant contribution to many aspects of modern plant biotechnology.

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References

  • Adaohambanasco, E. N.; Roscoe, D. H. Alginate—an alternative to agar in plant protoplast culture. Plant Sci. Lett 25:61–66; 1982.

    Article  Google Scholar 

  • Aditya, T. L.; Baker, D. A. Optimization of protoplast isolation from NaCl stressed primary, secondary and tertiary calli derived from mature seeds of Bangladeshi indica rice cultivar Binnatoa. Plant Growth Regul. 41:49–56; 2003.

    Article  CAS  Google Scholar 

  • Alayash, A. I. Oxygen therapeutics: can we tame hemoglobin? Nat. Rev. Drug Discov. 3:152–159; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Al-Forkan, M.; Anthony, P.; Power, J. B.; Davey, M. R.; Lowe, K. C. Haemoglobin (Erythrogen ™)-enhanced microcallus formation from protoplasts of indica rice (Oryza sativa L.). Artif. Cells Blood Substit. Immobil. Biotechnol. 29:399–404; 2002.

    Article  Google Scholar 

  • Anthony, P.; Davey, M. R.; Power, J. B.; Lowe, K. C. An improved protocol for the culture of cassava leaf protoplasts. Plant Cell Tiss. Organ Cult. 42:299–302; 1995.

    Article  CAS  Google Scholar 

  • Anthony, P.; Davey, M. R.; Power, J. B.; Lowe, K. C. Enhanced mitotic division of cultured Passiflora and Petunia protoplasts by oxygenated perfluorocarbon and haemoglobin. Biotechnol. Tech. 11:581–584; 1997a.

    Article  CAS  Google Scholar 

  • Anthony, P.; Davey, M. R.; Power, J. B.; Washington, C.; Lowe, K. C. Synergistic enhancement of protoplast growth by oxygenated perfluorocarbon and Pluronic F-68. Plant Cell Rep. 13:251–255; 1994.

    Article  CAS  Google Scholar 

  • Anthony, P.; Lowe, K. C.; Power, J. B.; Davey, M. R. Strategies for promoting division of cultured plant protoplasts: synergistic beneficial effects of haemoglobin (Erythrogen ™) and Pluronic ® F-68. Plant Cell Rep. 17:13–16; 1997b.

    Article  CAS  Google Scholar 

  • Assani, A.; Haicour, R.; Wenzel, G.; Foroughi-Wehr, B.; Bakry, F.; Cote, F. X.; Ducreux, G.; Ambroise, A.; Grapin, A. Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci. 162:355–362; 2002.

    Article  CAS  Google Scholar 

  • Augustynowicz, J.; Lekka, M.; Burda, K.; Gabrys., H. Correlation between chloroplast motility and elastic properties of tobacco mesophyll protoplasts. Acta Physiol. Plant 23:291–302; 2001.

    CAS  Google Scholar 

  • Azhakanandam, K.; Lowe, K. C.; Power, J. B.; Davey, M. R. Hemoglobin (Erythrogen ™)-enhanced mitotic division and plant regeneration from cultured rice protoplasts (Oryza sativa L.). Enzyme Microb. Technol. 21:572–577; 1997.

    Article  CAS  Google Scholar 

  • Bock, R. Transgenic plastids in basic research and plant biotechnology. J. Mol. Biol. 321:425–438; 2001.

    Article  CAS  Google Scholar 

  • Brewer, E. P.; Saunders, J. A.; Angle, J. S.; Chaney, R. L.; McIntosh, M. S. Somatic hybridization between the zinc accumulator Thlaspi caerulescens and Brassica napus. Theor. Appl. Genet. 99:761–771; 1999.

    Article  CAS  Google Scholar 

  • Carlson, P. S.; Smith, H. H.; Dearing, R. D. Parasexual interspecific plant hybridization. Proc. Natl Acad. Sci. USA 69:2292; 1972.

    Article  PubMed  Google Scholar 

  • Chen, L. P.; Zhang, M. F.; Xiao, Q. B.; Wu, J. G.; Hirata, Y. Plant regeneration from hypocotyl protoplasts of red cabbage (Brassica oleracea) by using nurse cultures. Plant Cell Tiss. Organ Cult. 77:133–138; 2004.

    Article  CAS  Google Scholar 

  • Cheng, M.; Lowe, B. A.; Spencer, T. M.; Ye, X. D.; Armstrong, C. L. Factors influencing Agrobacterium-mediated transformation of monoeotyle-donous species. In Vitro Cell. Dev. Biol. Plant 40:31–45; 2004.

    Article  Google Scholar 

  • Cheng, S. H.; Sheen, J.; Gerrish, C.; Bolwell G. P. Molecular identification of phenylalamine ammonia-lyase as a substrate of a constitutively active Arabidopsis CDPK expressed in maize protoplasts. FEBS Lett. 503:185–188; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Choi, S. K.; Choi, J. K.; Ryu, K. H. Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash. Virus Res 97:1–6; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Chupeau M. C.; Lemoine, M.; Chupeau, Y. Requirement of thidiazuron for healthy protoplast development to efficient tree regeneration of a hybrid Poplar (Populus tremula x Populus alba). J. Plant Physiol. 141:601–609; 1993.

    CAS  Google Scholar 

  • Cocking, E. C. A method for the isolation of plant protoplasts and vacuoles. Nature 187:927–929; 1960.

    Article  Google Scholar 

  • Commun, K.; Mauro, M. C.; Chupeau, Y.; Boulay, M.; Burrus, M.; Jeandet, P. Phytoalexin production in grapevine protoplasts during isolation and culture. Plant Physiol. Biochem. 41:317–323; 2003.

    Article  CAS  Google Scholar 

  • Cormeau, J.; Barthou, H.; Jauneau, A.; Petitprez, M.; Pont-Lezica, R.; Galaud, J. P. Cellular import of synthetic peptide using a cell-permeable sequence in plant protoplasts. Plant Physiol. Biochem. 40:1081–1086; 2002.

    Article  CAS  Google Scholar 

  • d'Utra Vaz, F. B.; Slamet, I. H.; Khatun., A.; Cocking, E. C.; Power, J. B. Protoplast culture in high molecular oxygen atmospheres. Plant Cell Rep. 11:416–418; 1992.

    Google Scholar 

  • Daniell, H.; Khan, M. S.; Allison, L. Milestones in chloroplastic genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci. 7:84–91; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Davey, M. R.; Anthony, P.; Power, J. B.; Lowe, K. C. Protoplast applications in biotechnology. In: Goodman, R. M., ed. Encyclopedia of plant and crop science. New York: Marcel Dekker Inc.: 2004:1061–1064.

    Google Scholar 

  • Davey, M. R.; Blackhall N. W.; Lowe, K. C.; Power, J. B. Stimulation of plant cell division and organogenesis by short-term, high-voltage electrical pulses. In: Lynch, P. T.; Davey, M. R., eds Electrical manipulation of cells. New York: Chapman and Hall; 1996:273–286.

    Google Scholar 

  • Davey, M. R.; Cocking, E. C.; Freeman, J. P.; Pearce, N.; Tudor, I. Transformation of Petunia protoplasts by isolated Agrobacterium plasmids. Plant Sci. Lett. 18:307–313; 1980.

    Article  CAS  Google Scholar 

  • Davey, M. R.; Kumar, A. Higher plant protoplasts—retrospect and prospect. Int. Rev. Cytol. Suppl. 16:219–299; 1983.

    CAS  Google Scholar 

  • Davey, M. R.; Marchant, R.; Power, J. B. Protoplasts of grain and forage legumes: their exploitation in genetic manipulation, physiological investigations and plant-pathogen interactions. In: Jaiwal, P. K.; Singh, R. P. eds. Improvement strategies for leguminosae biotechnology. Dordrecht: Kluwer Academic Publishers; 2003:133–153.

    Google Scholar 

  • Davey, M. R.; Power, J. B.; Lowe, K. C. Plant protoplasts. In: Spier, R. E. ed. Encyclopedia of cell technology. New York: John Wiley and Sons Inc.; 2000:1034–1042.

    Google Scholar 

  • Dijak, M.; Smith, D. L.; Wilson, T. J.; Brown, D. C. W. Stimulation of direct embryogenesis from mesophyll protoplasts of Medicago sativa. Plant Cell Rep. 5:468–470; 1986.

    Article  Google Scholar 

  • Dix, P. J.; Kavanagh, T. A. Transforming the plastome: genetic markers and DNA delivery systems. Euphytica 85:29–34; 1995.

    Article  CAS  Google Scholar 

  • Doi, R. H.; Tamaru, Y. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem. Rec. 1:24–32; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Dovzhenko, A.; Dal Bosco, C.; Meurer, J.; Koop, H.-U. Efficient regeneration from cotyledon protoplasts in Arabidopsis thaliana. Protoplasma 222:107–111; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Dovzhenko, A.; Koop, H-U. Sugarbeet (Beta vulgaris L.): shoot regeneration from callus and callus protoplasts. Planta 217:374–381; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Ephrussi, M. B.; Morel, M. G.; Tempé, M. J. Protoplastes et fusion de cellules somatiques végétales Colloques Internationaux de Centre National de la Recherche Scientifique no 212 Paris: Imprimerie Jouve; 1973.

    Google Scholar 

  • Eriksson, T. R. Protoplast isolation and culture. In: Fowke, L. C.; Constabel F., eds Plant protoplasts. Boca Raton, FL: CRC Press Inc.; 1985:1–20.

    Google Scholar 

  • Evans, D. A.; Bravo, J. E. Plant protoplast isolation and culture. Int. Rev. Cytol. Suppl. 16:33–53; 1983.

    CAS  Google Scholar 

  • Fan, L. M.; Wang, Y. F.; Wu, W. H. Outward K+ channels in Brassica chinensis pollen protoplasts are regulated by external and internal pH. Protoplasma 220:143–152; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Fleming, G. H.; Olivares-Fuster, O.; Del-Bosco, S. F.; Grosser, J. W. An alternative method for the genetic transformation of sweet orange. In Vitro Cell. Dev. Biol. Plant 36:450–455; 2000.

    Article  CAS  Google Scholar 

  • Folling, M.; Pedersen, C.; Olesen, A. Reduction of nuclease activity from Lolium protoplasts: effect on transformation frequency. Plant Sci. 139:29–40; 1998.

    Article  CAS  Google Scholar 

  • Frearson, E. M.; Power, J. B.; Cocking, E. C. The isolation, culture and regeneration of Petunia leaf protoplasts. Dev. Biol. 33:130–137; 1973.

    Article  PubMed  CAS  Google Scholar 

  • Gamborg, O. L. Plant tissue culture. Biotechnology. Milestones. In Vitro Cell. Dev. Biol. Plant 38:84–92; 2002.

    Article  Google Scholar 

  • Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.

    Article  PubMed  CAS  Google Scholar 

  • Golds, T. J.; Maliga, P.; Koop, H.-U. Stable transformation in PEG-treated protoplasts of Nicotiana tabacum Bio/Technology 11:95–97; 1993.

    Article  CAS  Google Scholar 

  • Goldsworthy, A. Electrostimulation of cells by weak electric currents. In: Lynch, P. T.; Davey, M. R. eds Electrical manipulation of cells. New York: Chapman and Hall; 1996:249–272.

    Google Scholar 

  • Gordon, K. H. J.; Williams, M. R.; Baker, J. S.; Gibson, J. M.; Bawden, A. L.; Millgate, A. G.; Larkin, P. J.; Hanzlik, T. N. Replication-independent assembly of an insect virus (tetraviridae) in plant cells. Virology 288:36–50; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Gummadi, S. N.; Panda, T. Purification and biochemical properties of microbial pectinases—a review. Process Boochem. 38:987–996; 2003.

    Article  CAS  Google Scholar 

  • Guo, W. W.; Deng, X. X.; Yi, H. L. Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding. Euphytica 116:281–285; 2000.

    Article  CAS  Google Scholar 

  • Hall, R. D.; Riksen-Bruinsma, T; Weyens, G. J.; Rosquin, I. J.; Denys, P. N.; Evans I. J.; Lathouwers, J. E.; Lefèbvre, M. P.; Dunwell, J. M.; van Tunen, A.; Krens, F. A. A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. Nature Biotechnol. 14:1133–1138; 1996.

    Article  CAS  Google Scholar 

  • Hasegawa, H.; Sato, M.; Suzuki, M. Efficient plant regeneration from protoplasts isolated from long-term, shoot primordial-derived calluses of garlie (Allium sativum). J. Plant Physiol. 159:449–452; 2002.

    Article  CAS  Google Scholar 

  • Hassanein, A. M.; Fayez, K. A.; Ahmed, A. M. Physiological studies on nuclear-organelle interaction of cybrid plants containing Solanum nigrum genome and Solanum tuberosum plastome. Phyton-Ann. REI Bot. 37:167–179; 1998.

    Google Scholar 

  • Hohe, A.; Reski, R. Optimisation of a bioreactor culture of the moss Physcomitrella patens for mass production of protoplasts. Plant Sci. 163:69–74; 2002.

    Article  CAS  Google Scholar 

  • Horita, M.; Moorhashi, H.; Komai, F. Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium x formolongi. Planta 217:597–601; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Ishikawa, S.; Bang, S. W.; Kaneko, Y.; Matsuzawa, Y. Production and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica aleracea. Plant Breed. 122:233–238; 2003.

    Article  Google Scholar 

  • Jain, R. K.; Khehra, G. S.; Lee, S.-H.; Blackhall, N. W.; Marchant, R.; Davey, M. R.; Power, J. B.; Cocking, E. C.; Gosal, S. S. An improved procedure for plant regeneration from indica and japonica rice protoplasts. Plant Cell Rep. 14:515–519; 1995.

    Article  CAS  Google Scholar 

  • Jansky, S.; Austin-Phillips, S.; McCarthy, C. Colorado potato beetle resistance in somatic hybrids of diploid interspecific Solanum clones. HortScience 34:922–927; 1999.

    Google Scholar 

  • Kao, K. N.; Constabel, F.; Michayluk, M. R.; Gamborg, O. L. Plant protoplast fusion and growth of intergeneric hybrid cells. Planta 120:215–227; 1974.

    Article  CAS  Google Scholar 

  • Kao, K. N.; Keller, W. A.; Miller, R. A. Cell division in newly formed cells from protoplasts of soybean. Exp. Cell Res. 62:338–340; 1970.

    Article  PubMed  CAS  Google Scholar 

  • Kao, K. N. Michayluk, M. R. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110; 1975.

    Article  CAS  Google Scholar 

  • Kim, H.; Mun, J. H.; Byun, B. H.; Hwang, H. J.; Kwon, Y. M.; Kim, S. G. Molecular cloning and characterization of the gene encoding osmotin protein in Petunia hybrida. Plant Sci. 162:745–752; 2002.

    CAS  Google Scholar 

  • Kim, S. W.; Oh, S. C.; In, D. S.; Liu, J. R. Plant regeneration of rose (Rosa hybrida) from embryogenic cell-derived protoplasts. Plant Cell Tiss. Organ Cult. 73:15–19; 2003.

    Article  CAS  Google Scholar 

  • Kisaka, H.; Kisaka, M.; Kanno, A.; Kameya, T. Intergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion. Plant Cell Rep. 17:362–367; 1998.

    Article  CAS  Google Scholar 

  • Klaus, S. M. J.; Huang, F-C.; Eibl, C.; Koop, H-U; Golds, T. J. Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis. Plant J. 35:811–821; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Klereker, J. A. F. Eme methode zur isolierung lebender protoplasten. Ofvers. Vetensk. Akad. Forh. Stokh. 9:463–475; 1892.

    Google Scholar 

  • Kochevenko, A.; Willmitzer, L. Chimeric RNA/DNA oligonucleotide-based site-specific modification of the tobacco acctolactate synthase gene. Plant Physiol. 132:174–184; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Kopp, H-U; Steimmuller, K.; Wagner, H.; Rossler, C.; Eibl, C.; Sacher, L. Integration of foreign sequences into the tobacco plastome yia polyethylene glycol-mediated protoplast transformation. Planta 199:193–201: 1996.

    Google Scholar 

  • Krasnyanski, S.; Ball, T. M.; Sink, K. C. Somatic hybridization in mint: identification and characterization of Mentha piperata (+) M. spicata hybrid plants. Theor. Appl. Genet. 96:683–687; 1998.

    Article  Google Scholar 

  • Krasnyanski, S.; May, R. A.; Loskutov, A.; Ball, T. M.; Sink, K. C. Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants. Theor. Appl. Genet. 99:676–682; 1999.

    Article  CAS  Google Scholar 

  • Krens, F. A.; Molendijk, L.; Wullems, G. J.; Schilperoort, R. A. In vitro transformation of plant protoplasts with Ti plasmid DNA. Nature 296:72–74; 1982.

    Article  CAS  Google Scholar 

  • Kumar, V.; Laouar, L.; Davey, M. R.; Mulligan, B. J.; Lowe, K. C. Pluronic F-68 stimulates growth of Solanum dulcamara in culture. J. Exp. Bot. 43:487–493; 1992.

    Article  CAS  Google Scholar 

  • Küster, E. Über die Verschnelzung nachter Protoplasten. Ber. Dtsch. Bot. Ges. 27:589–598; 1909.

    Google Scholar 

  • Liu, B.; Liu, Z. L.; Li, X. W. Production of highly asymmetric somatic hybrid between rice and Zizania latifolia (Griseb): evidence for intergenomic exchange. Theor. Appl. Genet. 98:1099–1103; 1999.

    Article  Google Scholar 

  • Liu, J. H.; Deng, X. X. Regeneration and characterization of plants derived from asymmetric protoplast fusion in Citrus. Acta Bot. Sin. 42:1144–1149; 2000.

    Google Scholar 

  • Lörz, H.; Larkin, P. L.; Thomson, L.; Scowcroft, W. R. Improved protoplast culture and agarose media. Plant Cell Tiss. Organ Cult. 2:217–226; 1983.

    Article  Google Scholar 

  • Lowe, K. C. Engineering blood synthetic substitutes from fluorinated compounds. Tissue Engng 9:389–399; 2003.

    Article  CAS  Google Scholar 

  • Lowe, K. C.; Anthony, P.; Davey, M. R.; Power, J. B. Culture of cells at perfluorocarbon-aqueous interfaces. Artif. Cells Blood Substit. Immobil. Biotechnol. 27:255–261; 1999.

    PubMed  CAS  Google Scholar 

  • Lowe, K. C.; Anthony, P.; Davey, M. R. Beneficial effects of Pluronic F-68 and artificial oxygen carriers on the post-thaw recovery of cryopreserved plant cells. Artif. Cells Blood Substit. Immobil. Biotechnol. 29:297–316; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Lowe, K. C.; Anthony, P.; Power, J. B.; Davey, M. R., Novel approaches for regulating gas supply to plant systems in vitro: application and benefits of artificial gas carriers. In Vitro Cell. Dev. Biol. Plant 39:557–566; 2003.

    Article  CAS  Google Scholar 

  • Lowe, K. C.; Davey, M. R.; Power, J. B. Perfluorochemicals: their applications and benefits to cell culture. Trends Biotechnol. 16:272–277; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Lowe, K. C.; Davey, M. R.; Power, J. B.; Clothier, R. H. Plants as toxicity screens. Pharm. News 2:17–22; 1995.

    Google Scholar 

  • Lynch, P. T.; Davey, M. R. Electrical manipulation of cells. New York: Chapman and Hall: 1996.

    Google Scholar 

  • Ma, R.; Guo, Y. D.; Pulli, S. Somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye (Secale cereale L.). Plant Cell Rep., 22:320–327; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Maddumage, R.; Fung, R. M. W.; Ding, H.; Simons, J. L.; Allan, A. C. Efficient transformation of suspension culture-derived apple protoplasts. Plant Cell Tiss Organ Cult. 70:77–82; 2002.

    Article  CAS  Google Scholar 

  • Maliga, P. Progress towards commercialisation of plastid transformation technology. Trends Biotechnol. 21:20–28; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Maliga, P. Plastid transformation in higher plants. Annu. Rev. Plant Biol. 55:289–313; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Martens, S.; Teeri, T.; Forkmann, C. Heterologous expression of dihydroflanonol 4-reductases from various plants. FEBS Lett. 531:453–458; 2002.

    Article  PubMed  CAS  Google Scholar 

  • May, R. A.; Sink, K. C. Genotype and auxin influence direct somatic embryogenesis from protoplasts derived from embryogenic-cell suspensions of Asparagus officinalis L. Plant Sci. 108:71–84; 1995.

    Article  CAS  Google Scholar 

  • Mendes-da-Gloria, F. J.; Mourao, F. D.; Camargo, L. E. A.; Mendes, B. M. J. Caipira sweet organe plus Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry. Genet. Mol. Biol. 23:661–665; 2000.

    Article  Google Scholar 

  • Morse, M.; Pironcheva, G.; Gehring, C. AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation. FEBS Lett. 556:99–103; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Motegi, T.; Nou, I. S.; Zhou, J.; Kanno, A.; Kameya, T.; Hirata, Y. Obtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile). Euphytica 129:319–323; 2003.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Nagata, T.; Takebe, I. Plating of isolated tobacco-mesophyll protoplasts on agar medium. Planta 99:12–20; 1971.

    Article  Google Scholar 

  • Nakano, M.; Tanaka, S.; Oota, M.; Ookawa, E.; Suzuki, S.; Saito, H. Regeneration of diploid and tetraploid plants from callus derived from protoplasts of Agapanthus praecox ssp. orientalis (Leighton) Leighton. Plant Cell Tiss. Organ Cult. 72:63–69; 2003.

    Article  CAS  Google Scholar 

  • Nassour, M.; Chasseriaux, G.; Dorion, N. Optimization of protoplast-to-plant system for Pelargonium × hortorum ‘Alain’ and genetic stability of the regenerated plants. Plant Sci. 165:121–128; 2003.

    Article  CAS  Google Scholar 

  • Ochatt, S. J.; Chand, P. K.; Rech, E. L.; Davey, M. R.; Power, J. B. Electroporation-mediated improvement of plant regeneration from Colt cherry (Prunus arium × pseulocerasus) protoplasts. Plant Sci. 54:165–169; 1988.

    Article  Google Scholar 

  • Oh, M.-H.; Clouse, S. D. Brassinolide affects the rate of cell division in isolated leaf protoplasts of Petunia hybrida. Plant Cell Rep. 17:921–924; 1998.

    Article  CAS  Google Scholar 

  • Olivares-Fuster, O.; Pena, L.; Duran-Vila, N.; Navarro, L. Green fluorescent protein as a visual markar in somatic hybridization. Ann. Bot. 89:491–497; 2002.

    Article  PubMed  CAS  Google Scholar 

  • O'Neill C.; Horváth, G. V.; Horváth, E.; Dix, P. J.; Medgyesy, P. Chloroplast transformation in plants: polyethylene glycol (PEC) treatment of protoplasts is an alternative to biolistic delivery systems. Plant J. 3:729–738; 1993.

    Article  PubMed  Google Scholar 

  • Orezyk, W.; Przetakiewicz, J.; Nadolska-Orczyk, A. Somatic hybrids of Solanum tuberosum—application to genetics and breeding. Plant Cell Tiss. Organ Cult. 74:1–13; 2003.

    Article  Google Scholar 

  • Ortin-Parraga, E.; Burgos, I.: Isolation and culture of mesophyll protoplasts from apricot. J. Hort. Sci. Biotechnol. 78:624–628; 2003.

    Google Scholar 

  • Pandey, S.; Wang, X. Q.; Coursol, S. A.; Assmann, S. M. Preparation and applications of Arabidopsis thaliana guard cell protoplasts. New Phytol. 153:517–526: 2002.

    Article  CAS  Google Scholar 

  • Papadakis, A. K.; Roubelakis-Angelakis, K. A. Oxidative stress could be responsible for the recalcitrance of plant protoplasts. Plant Physiol. Biochem. 40:519–559; 2002.

    Article  Google Scholar 

  • Partanen, C. R. Filter papers as a support and carrier for plant protoplast cultures. In Vitro 17:77–80; 1981.

    Google Scholar 

  • Pasternak, T.; Miskolezi, P.; Ayaydin F.; Meszaros, T.; Dudits, D.; Fehér, A. Exogenous auxin and cytokinin dependent activation of CDKs and cell division in leaf protoplast-derived cells of alfalfa. Plant Growth Regul. 32:129–1412: 2000.

    Article  CAS  Google Scholar 

  • Potrykus, I.; Harms, C. T.; Hinnen A.; Hütter, R.; King, P. J.; Shillito, R. D. Protoplasts 1983 Lecture. Proc. 6th Int. Protoplast Symp. Basel, August 12–16. 1983. Experientia Suppl. 46. Basel: Birkluser Verlag: 1983.

    Google Scholar 

  • Potrykus, I.; Harms, C. T.; Lörz, H. Multiple-droparray (MDA) technique for the large-scale testing of culture media variations in hanging drop microdrop cultures of single cell systems I. The technique. Plant Sci. Lett. 14:231–235; 1979.

    Article  Google Scholar 

  • Power, J. B.; Cocking, E. C. Isolation of leaf protoplasts: macromolecular uptake and growth substance response. J. Exp. Bot. 21:64–70; 1970.

    Article  CAS  Google Scholar 

  • Power, J. B.; Cummins, S. E.; Cocking, E. C. Fusion of isolated plant protoplasts. Nature 225:1016; 1970.

    Article  PubMed  CAS  Google Scholar 

  • Power, J. B.; Davey, M. R.; Anthony, P.; Lowe, K. C. Protoplast culture and regeneration. In: Goodman, R. M., ed. Encyclopedia of plant and crop science. New York: Marcel Dekker Inc.; 2004:1065–1068.

    Google Scholar 

  • Power, J. B.; Davey, M. R.; Sadia, B.; Anthony, P.; Lowe, K. C. Hacmoglobin-enhanced mitosis in cultured plant protoplasts. Adv. Exp. Med. Biol. 540:201–206; 2003.

    PubMed  CAS  Google Scholar 

  • Qian, W.; Liu, R.; Meng, J.: Genetic effects on biomass yield in interspecific hybrids between Brassica napus and B. rapa. Euphytica 134:9–15:, 2003.

    Article  CAS  Google Scholar 

  • Rakoczy-Trojanowska, M. Alternative methods of plant transformation—a short review. Cell. Mol. Biol. Lett. 7:849–858; 2002.

    PubMed  Google Scholar 

  • Raveh, D.; Huberman, E.; Galun, E. In vitro culture of tobacco protoplasts: use of feeder techniques to support division of cells plated at low densities. In Vitro, 9:216–222; 1973.

    PubMed  CAS  Google Scholar 

  • Rech, E. L.; Ochatt, S. J.; Chaud, P. K., Power, J. B.; Davey, M. R. Electroenhancement of division of plant protoplast-derived cells. Protoplasma 141:169–176: 1987.

    Article  Google Scholar 

  • Ruf, S.; Hermann, M.; Berger, I. J.; Carrer, H.; Bock, R. Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nature Biotechnol 19:870–875; 2001.

    Article  CAS  Google Scholar 

  • Sairam, R. V.; Seetharama, N.; Devi, P. S.; Verma, A.; Murthy, U. R.; Potrykus, I. Culture and regeneration of mesophyll-derived protoplasts of sorghum (Sorghum bicolour L. Meonch). Plant Cell Rep. 18:927–977: 1999.

    Article  Google Scholar 

  • Sato, H.; Yamada, K.; Mii, M.; Hosomi, K.; Okuyama, S.; Uzawa, M.; Ishikawa, H.; Ito, Y. Production of an interspecific somatic hybrid between peppermint and gingermint. Plant Sci. 115:101–107; 1996.

    Article  CAS  Google Scholar 

  • Sawabe, T.; Ezura, Y.; Yamamoto, H. Plant regereration from protoplasts of Laminaria japonica Areschoug (Laminariales. Phaeophyceae) in a continuous-flow culture system. Plant Cell Rep. 17:109–111; 1997.

    Article  CAS  Google Scholar 

  • Schillberg, S.; Fischer, R.; Emans, N. Molecular farming of recombinant antibodies in plants. J. Cell. Mol. Life Sci. 60:433–445; 2003.

    Article  CAS  Google Scholar 

  • Schween, G.; Hohe, A.; Koprivova, A.; Reski, R. Effects of nutrients, cell density and culture techniques on protoplast regeneration and early protonema development in a moss, Physcomitrella patents J. Plant Physiol. 160:209–212; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Sheen, J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 127:1466–1475; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Shillito, R. D.; Saul, M. W.; Paszkowski, J.; Müller, M.; Potrykus, I. High efficiency direct gene transfer to plants. Bio/Technology 3:1099–1103; 1985.

    Article  Google Scholar 

  • Sinha, A.; Wetten, A. C.; Caligari, P. D. S. Effect of biotic factors on the isolation of Lupinus albus protoplasts. Austr. J. Bot. 51:103–109; 2003.

    Article  Google Scholar 

  • Steffens, B.; Feckler, C.; Palme, K.; Christian, M.; Bottger, M.; Luthen, H. The auxin signal for protoplast swelling is perceived by extracellular ABPI. Plant J. 27:591–599; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Stegemann, S.; Hartmann, S.; Ruf, S.; Bock, R. High-frequency gene transfer from the chloroplast genome to the nucleus. Proc. Natl Acad. Sci. USA 100:8828–8833; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Sugimoto, K.; Lidbetter, J. Plant regeneration from mesophyll protoplasts of Goodenia scaerolina F. Muell. J. Jpn Soc. Hort. Sci. 72:281–285; 2003.

    CAS  Google Scholar 

  • Svab, Z.; Maliga, P. High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc. Natl Acad. Sci. USA 90:913–917; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Takebe, L.; Labib, G.; Melchers, G. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58:318–320; 1971.

    Article  Google Scholar 

  • Takebe, L.; Otsuki Y.; Aoki, S. Isolation of tobacco mesophyll protoplasts in intact and active state. Plant Cell Physiol. 9:115–124; 1968.

    Google Scholar 

  • Takeda, Y.; Kasamo, K. Transmembrane topography of plasma membrane constituents in mung bean (Vigna radiata L.) hypocotyl cells–1 Transmembrane distribution of phospholipids. Biochim. Biophys. Acta Biomembr. 1513:38–48; 2001.

    Article  CAS  Google Scholar 

  • Tang, T.; Sun, X.; An, D.; Power, J. B.; Cocking, E. C.; Davey, M. R.. A simple and rapid procedure to establish embryogenic cell suspensions as a source of protoplasts for efficient plant regeneration from two Chinese commercial rice cultivars. Plant Cell Tiss. Organ Cult. 66:149–153; 2001.

    Article  CAS  Google Scholar 

  • Tylicki, A.; Burza, W.; Malepszy S.; Kuras, M. Changes in the organization of the tubulin cytoskeleton during the early stages of Solanum lycopersicoides Dun. protoplast culture. Plant Cell Rep. 22:312–319; 2003.

    Article  PubMed  CAS  Google Scholar 

  • VazquezTello, A.; Yang, L. J.; Hidaka, M.; Uozumi, T. Inherited chilling tolerance in somatic hybrids of transgenic Hibiscus rosa-sinensis × transgenic Lavalera thuringiaca selected by double-antibiotic resistance. Plant Cell Rep. 15:506–511; 1996.

    Article  Google Scholar 

  • Vishnichenko, V. K.; Zavriev, S. K. Detection of infections viral particles in plant protoplasts inoculated with transcripts of full-length shallot virus X cDNA—brief report Arch. Virol. 146:1213–1217; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Wallin, A.; Glimelius, K.; Eriksson, T. Induction of aggregation and fusion of Daucus carota protoplasts by polyethylene-glycol. Z. Pflanzenphysiol. 74:64–80; 1974.

    CAS  Google Scholar 

  • Wardrop, J.; Davey, M. R.; Power, J. B.; Lowe, K. C. Beneficial effects of oxygenated fluorocarbon on the in vitro culture of protoplasts and cell electrofusion products. Artif. Cells Blood Substit. Immobil. Biotechnol. 25:481–486; 1997a.

    PubMed  CAS  Google Scholar 

  • Wardrop, J.; Edwards, C. M.; Lowe, K. C.; Davey, M. R.; Power, J. B. Changes in cell biochemistry in response to culture of protoplasts with oxygenated perfluorocarbon. Artif. Cells Blood Substit. Immobil. Biotechnol. 25:585–589; 1997b.

    PubMed  CAS  Google Scholar 

  • Wardrop, J.: Lowe, K. C.; Power, J. B.; Davey, M. R. Perfluorochemicals and plant biotechnology: an improved protocol for protoplast culture and plant regeneration in rice (Oryza sativa L.). J Biotechnol. 50:47–54; 1996.

    Article  CAS  Google Scholar 

  • Watanabe, M.; Suzuki, K.; Kawasaki, H.; Watanabe, Y. Differential responses of Brassica napus and Petunia hybrida to leaf protoplast isolation stress. Physiol. Plant 114:645–651; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Widholm, J. The use of FDA and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47:186–194; 1972.

    Google Scholar 

  • Winfield, S.; Lawton, R.; Daniell, H.; Dhir, S. K. Transformation of sweet potato tissues with green-fluorescent protein gene. In Vitro Cell. Dev. Biol. Plant 37:648–653; 2001.

    Article  CAS  Google Scholar 

  • Xia, G. M.; Xiang, F. N.; Zhou, A. F.; Wang, H.; He, S. X.; Chen, H. M. Fertile hybrid plant regeneration from somatic hybridization between Triticum aestivum and Agropyron elongatum. Acta Bot. Sin. 41:349–352; 1999.

    Google Scholar 

  • Xiang, F. N.; Xia, G. M.; Zhou, A. F.; Chen, H. M.; Huang, Y.; Zhai, X. L. Asymmetric somatic hybridization between wheat (Triticum aestivum) and Bromus inermis. Acta Bot. Sin. 41:458–462; 1999.

    Google Scholar 

  • Xu, X.; Liu, J.; Deng, X. Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour organe (Citrus aurantium). Euphytica 136:115–123; 2004.

    Article  Google Scholar 

  • Yoshida, K.; Shinmyo, A. Transgene expression systems in plant, a natural bioreactor. J. Biosci. Bioengng 90:353–362; 2000.

    CAS  Google Scholar 

  • Zhao, J.; Mollet J. C.; Lord, E. M. Lily (Lilium longiflorum L.) pollen protoplast adhesion is inereased in the presence of the peptide SCA. Sex. Plant. Reprod. 16:227–233; 2004.

    Article  CAS  Google Scholar 

  • Zhao, J.; Morozova, N.; Williams, L.; Avivi Y.; Grafi, G. Two phases of chromatin decondensation during dedifferentiation of plant cells–distinction between competence for cell fate switch and a commitment for S phase. J. Biol. Chem. 276:22772–22778; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Zhou, A. F.; Xia, G. M.; Chen, H. M.; Hu, H. Comparative study of symmetric and asymmetric somatic hybridization between common wheat and Haynaldia villosa. Sci. China Ser. C Life Sci. 44:294–304; 2001.

    Google Scholar 

  • Zimmermann, U.; Scheurich, P. High, frequency fusion of plant protoplasts by electrical fields. Planta 151:26–32; 1981.

    Article  Google Scholar 

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Authors and Affiliations

  1. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Longhborough, UK

    Michael R. Davey, Paul Anthony & J. Brian Power

  2. School of Biology, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    Kenneth C. Lowe

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  1. Michael R. Davey
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  2. Paul Anthony
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  3. J. Brian Power
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  4. Kenneth C. Lowe
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Correspondence to Michael R. Davey.

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Davey, M.R., Anthony, P., Power, J.B. et al. 2004 SIVB Congress Symposium Proceedings “Thinking Outside the Cell”: Plant Protoplast Technology: Status and Applications. In Vitro Cell.Dev.Biol.-Plant 41, 202–212 (2005). https://doi.org/10.1079/IVP2004633

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