Abstract
Plant regeneration was achieved from plumules excised from mature zygotic embryos of a local coconut cultivar (Sri Lanka Tall). A detailed histological study was undertaken to gain a better understanding of the cellular changes that occur during plant regeneration from plumule tissues. This study led to the identification of the cellular origin, specific cell characterization and development pattern of embryogenic calluses. It also revealed that abscisic acid induces plant regeneration through somatic embryogenesis. The presence of incomplete somatic embryos that lacked shoot poles was also observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buffard-Morel J, Verdeil JL & Pannetier C (1992) Embryogenesis somatique du cocotier (Cocos nucifera L.) a partir de tissus foliaires: etude histologique. Can. J. Bot. 70: 735-741
Chan JL, Saenz L, Talavera C, Hornung R, Robert M & Oropeza C (1998) Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep. 17: 515-521
Eeuwens CJ (1978) Effects of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera) and date (Phoenix dactylifera) palms cultured in vitro. Physiol. Plant. 42: 173-178
Fernando SC & Gamage CKA (2000) Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos nucifera L.). Plant Sci. 151: 193-198
Hornung R (1995) Micropropagation of Cocos nucifera L. from plumular tissues excised from mature zygotic embryos. Planta-tions Res. Dev. 2: 38-41
Karunaratne S & Periyapperuma K (1989) Culture of immature embryos of coconut, Cocos nucifera L: callus proliferation and somatic embryogenesis. Plant Sci. 62: 247-253
Samosir YMS, Godwin ID & Adkins SW (1999) The use of osmotically active agents and abscisic acid can optimize the maturation of coconut somatic embryos. In: Oropeza C, Verdeil JL, Ashburner GR, Cardena R & Santamaria JM (eds) Current Advances in Coconut Biotechnology (pp 341-354). Kluwer Academic Publishers, Dordrecht
Schwendiman J, Pannetier C & Michaux-Ferriere N (1988) Histol-ogy of somatic embryogenesis from leaf explants of the oil palm Elaeis guineensis. Ann. Bot. 62: 41-52
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fernando, S., Verdeil, J., Hocher, V. et al. Histological analysis of plant regeneration from plumule explants of Cocos nucifera . Plant Cell, Tissue and Organ Culture 72, 281–283 (2003). https://doi.org/10.1023/A:1022345011002
Issue Date:
DOI: https://doi.org/10.1023/A:1022345011002