Abstract
Purpose. The general properties of micellar electrokinetic capillary chromatography (MECC) were utilized to characterize the strength of interactions between bile salts and biological active substances.
Methods. For that purpose various bile salts were used as micellar pseudostationary phase in the background electrolyte. Furthermore, a physicochemical model was applied and the effective partition coefficients between micellar and water phase were calculated in order to evaluate the strength of interactions between bile acids and the drugs.
Results. It was found that the interactions between the selected drugs and bile salts depend both on the lipohilicity of the drugs and on the charge of the components. Only hydrophobic, cationic drugs such as quinine and propranolol are able to interact with these surface-active agents.
Conclusions. MECC is a valuable methode to characterize interactions such occurring between drugs and bile salts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
R. Neubert, B. Fritsch, and G. Dongowski. Wechselwirkungen zwischen Nahrungsbestandteilen und Arzneistoffen. Pharmazie 48:723–728 (1993).
M. R. Gasco, M. Trotta, and M. Eandi. The influence of bile salts on the adsorption in vitro and in vivo of propranolol. J. Pharm. & Biomed. Anal. 2:425–439 (1984).
F. Poelma, J. Tukker, and D. Crommelin. The role of bile salts in the intestinal absorption of drug. Acta Pharm. Technol. 36(29):43–52 (1990).
D. M. Small, In: The Bile Acids (P. P. Nair, D. Krichevsky, Eds.) 249–356, Plenum Press, New York (1971).
N. A. Mazer, M. C. Carey, R. F. Kwasnick, and G. B. Benedek. Quasielastic light scattering studies of aqueous biliary lipid system. Size, shape, and thermodynamics of bile salts. Biochemistry 18:3064–3075 (1979).
S. Terabe, M. Shibata, and Y. Miyashita. Chiral separation by electrokinetic chromatography with bile salt micelles. J. Chromatogr., 480, 403–411 (1989).
H. Nishi, T. Fukuyama, and M. Matsuo. Chiral separation of optical isomeric drugs using micellar electrokinetic chromatography and bile salts. J. Microcolumn Sep. 1:234–41 (1989).
R. Cole, M. Sepaniak, W. Hinze, J. Gorse, and K. Oldiges. Bile salt surfactants in micellar electrokinetic capillary chromatography: application to hydrophobic molecule separation. J. Chromatogr. 557:114–23 (1991)
Ch. Bjergegaard, H. Simonsen, and H. Sorensen. Determination of heterocyclic compounds by micellar electrokinetic capillary chromatography. J. Chromatogr. 680:561–569 (1994)
S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, and T. Ando. Electrokinetic separations with micellar solution and open-tubular capillaries. Anal. Chem. 56:111–113 (1984)
S. Terabe, K. Otsuka, and T. Ando. Electrokinetic chromatography with micellar solution and open-tubular capillary. Anal. Chem. 57:834–841 (1985)
M. Jannich, M. Schwarz, R. Neubert, in preparation
D. M. Small, In: The Bile Acids (P. P. Nair, D. Krichevsky, Eds.) 203–226, Plenum Press, New York (1971)
H. Hoffmann, and W. Ulbricht. Transition of rodlike to globular micelles by the solubilization of additives. J. Colloid Interface Sci. 129:388 (1989)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schwarz, M.A., Neubert, R.H.H. & Dongowski, G. Characterization of Interactions Between Bile Salts and Drugs by Micellar Electrokinetic Capillary Chromatography. Part I.. Pharm Res 13, 1174–1180 (1996). https://doi.org/10.1023/A:1016051917608
Issue Date:
DOI: https://doi.org/10.1023/A:1016051917608