Abstract
The electro-optical characteristics of suspensions of Escherichia coli K-12 cells metabolizing glucose, lactose, and galactose were studied by measuring the suspension turbidity as a function of cell alignment in an orienting electric field whose frequency was varied from 10 kHz to 10 MHz. In a frequency range of 10 kHz to 1 MHz, the orientational spectra of E. coli K-12 cells grown on glucose and lactose considerably changed after their incubation in the presence of the sugars. These changes likely reflect alterations in the polarizability of the cells induced by sugar metabolism.
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REFERENCES
Ignatov, O.V., Khorkina, N.A., Shchyogolev, S.Yu., Khlebtsov, N.G., Rogacheva, S.M., and Bunin, V.D., Electro-Optical Properties of Microbial Cells as Affected by Acrylamide Metabolism, Anal. Chim. Acta, 1997, vol. 347, pp. 241–247.
Ignatov, O.V., Khorkina, N.A., Tsivilyova, O.N., Bunin, V.D., Shchyogolev, S.Yu., and Ignatov, V.V., Utilization of an Electro-Optical Method to Investigate the Amidase Activity of Microbial Cells, FEMS Microbiol. Lett., 1998, vol. 162, pp. 105–110.
Ignatov, O.V., Khorkina, N.A., Singirtsev, I.N., Bunin, V.D., and Ignatov, V.V., Exploitation of the Electro-Optical Characteristics of Microbial Suspensions for Determining p-Nitrophenol and Microbial Degradative Activity, FEMS Microbiol. Lett., 1998, vol. 165, pp. 301–304.
Ignatov, O.V., Khorkina, N.A., Shchyogolev, S.Yu., Singirtsev, I.N., Bunin, V.D., Tumaikina, Y.A., and Ignatov, V.V., A Comparison of the Electro-Optical Properties and Specific Respiratory Activity of Acinetobacter calcoaceticum A-122, FEMS Microbiol. Lett., 1999, vol. 173, pp. 453–457.
Ignatov, O.V., Khorkina, N.A., Shchyogolev, S.Yu., Singirtsev, I.N., Bunin, V.D., Tumaikina, Y.A., and Ignatov, V.V., An Investigation into the Electro-Physical Characteristics of Microbial Cells during the Metabolism of Toxic Compounds under Conditions of Limited O2 Availability, FEMS Microbiol. Lett., 1999, vol. 178, pp. 259–264.
Lehninger, A.L., Principles of Biochemistry, New York: Worth Publ., 1982. Translated under the title Osnovy biokhimii, Moscow: Mir, 1985.
Gottschalk, G., Bacterial Metabolism, Berlin: Springer, 1979. Translated under the title Metabolizm bakterii, Moscow: Mir, 1982.
Khlebtsov, N.G., Melnikov, A.G., and Bogatyrev, V.A., The Linear Dichroism and Birefringence of Colloidal Dispersions: Approximate and Exact Approaches, J. Colloid Interface Sci., 1991, vol. 146, pp. 463–478.
Bottcher, C.F., Theory of Electrical Polarizability, New York: Academic, 1978.
Shchyogolev, S.Yu., Khlebtsov, N.G., Bunin, V.D., Sirota, A.I., and Bogatyrev, V.A., Inverse Problems of Spectroturbidimetry of Biological Disperse Systems with Random and Ordered Particle Orientation, Proc. SPIE, 1994, vol. 2082, pp. 167–176.
Khlebtsov, N.G., Bogatyrev, V.A., Sirota, A.I., and Mel'nikov, A.G., The Dipole Moment of Bacterial Cells, Biofizika, 1990, vol. 35, p. 173.
Bunin, V.D. and Voloshin, A.G., Determination of Cell Structures, Electrophysical Parameters, and Cell Population Heterogeneity, J. Colloid Interface Sci., 1996, vol. 180, pp. 122–126.
Khimicheskaya entsiklopediya (Chemical Encyclopedia), Knunyants, I.A. et al., Eds., Moscow: Sov. Entsikl., 1988, p. 623.
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Ignatov, O.V., Gribanova, Y.S., Shchegolev, S.Y. et al. The Electro-Optical Investigation of Suspensions of Escherichia coli K-12 Cells Metabolizing Glucose, Lactose, and Galactose. Microbiology 71, 302–306 (2002). https://doi.org/10.1023/A:1015854611552
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DOI: https://doi.org/10.1023/A:1015854611552