Abstract
Pathotype-specific and broad-spectrum resistance to turnip mosaic virus (TuMV) have been identified in the diploid A genome brassica species Brassica rapa. The pathotype-specific resistance is effective against pathotype 1 isolates of TuMV, which are the most common in Europe. It is almost identical in its specificity to that of a mapped resistance gene (TuRB01) present in the A genome of the amphidiploid species Brassica napus. A mutant of a pathotype 1 isolate of TuMV (UK 1M) that is able to overcome TuRB01 also overcame the B. rapa resistance. This, combined with the fact that a single-nucleotide mutation in the cylindrical inclusion gene of TuMV that has been shown to induce a change from avirulence to virulence against TuRB01, had an identical effect on the B. rapa resistance, suggest that the two resistances are conditioned by the same gene. A second source of resistance in B. rapa prevented systemic spread of all TuMV isolates tested. A third source of resistance that appears to provide immunity to, or severely restrict replication of most isolates of TuMV has been characterised. This resistance source also prevented systemic spread of all TuMV isolates tested. Prior to this study, no resistance to pathotype 4 or pathotype 12 isolates of TuMV had ever been identified. For each of these three resistance sources, plant lines that are not segregating for some of the resistance phenotypes and that are presumably homozygous for the genes controlling these phenotypes have been generated. Strategies for further characterising and deploying these resistances in different Brassica species are described.
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Chiang BY, Chiang MS, Grant WF and Crete R (1980) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea ssp. capitata). IV. A resistant 18-chromosome B1 plant and its B2 progenies. Euphytica 29: 47–55
Evans IR and MacNeil BH (1983) Virus disease of rutabagas (turnips). Ontario Ministry of Agriculture and Food Factsheet 73-061
Green SK and Deng TC (1985) Turnip mosaic virus strains in cruciferous hosts in Taiwan. Plant Disease 69: 28–31
Jenner CE and Walsh JA (1996) Pathotypic variation in turnip mosaic virus with special reference to European isolates. Plant Pathology 45: 848–856
Jenner CE, Keane GJ, Jones JE and Walsh JA (1999) Serotypic variation in turnip mosaic virus. Plant Pathology 48: 101–108
Jenner CE, Sánchez F, Nettleship SB, Foster GD, Ponz F and Walsh JA (2000) The cylindrical inclusion gene of turnip mosaic virus encodes a pathogenic determinant to the Brassica resistance gene TuRB01. Molecular Plant-Microbe Interactions 13: 1102–1108
Lehmann P, Petrzik K, Jenner C, Greenland A, Spak J, Kozubek E and Walsh JA (1997) Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance gene TuRB01. Physiological and Molecular Plant Pathology 51: 195–208
Liu XP, Lu WC, Liu YK, Wei SQ, Xu JB, Liu JB, Liu ZR, Zhang HJ, Li JL, Ke GL, Yao WY, Cai YS, Wu FY, Cao SC, Li YH, Xie SD, Lin BX and Zhang CL (1996) Occurrence and strain differentiation of turnip mosaic potyvirus and sources of resistance in Chinese cabbage in China. Acta Horticulturae 407: 431–440
Niu X, Leung H and Williams PH (1983) Sources and nature of resistance to downy mildew and turnip mosaic virus in Chinese cabbage. Journal of the American Society for Horticultural Science 108: 775–778
Provvidenti R (1980) Evaluation of Chinese cabbage cultivars from Japan and the People's Republic of China for resistance 20 to turnip mosaic virus and cauliflower mosaic virus. Journal of the American Society for Horticultural Science 105: 571–573
Sánchez F, Martínez Herrera D, Aguilar I and Ponz F (1998) Infectivity of turnip mosaic potyvirus cDNA clones and transcripts on the systemic host Arabidopsis thaliana and local lesion hosts. Virus Research 55: 207–219
Shattuck VI (1992) The biology, epidemiology and control of turnip mosaic virus. In: Janick J (ed) Plant Breeding Reviews. Vol. 14 (pp 199–238) John Wiley and Sons, New York
Shattuck VI and Stobbs LW (1987) Evaluation of rutabaga cultivars for turnip mosaic virus resistance and the inheritance of resistance. HortScience 22: 935–937
Suh SK, Green SK and Park HG (1995) Genetics of resistance to five strains of turnip mosaic virus in Chinese cabbage. Euphytica 81: 71–77
Tomlinson JA (1987) Epidemiology and control of virus diseases of vegetables. Annals of Applied Biology 110: 661–681
Tomlinson JA and Ward CM (1978) The reactions of swede (Brassica napus) to infection by turnip mosaic virus. Annals of Applied Biology 89: 61–69
Walsh JA (1986) Viruses in oilseed rape, a potential threat to vegetable crops. Aspects of Applied Biology 12: 59–68
Walsh JA (1989) Genetic control of immunity to turnip mosaic virus in winter oilseed rape (Brassica napus ssp. oleifera) and the effect of foreign isolates of the virus. Annals of Applied Biology 115: 89–99
Walsh JA, Sharpe AG, Jenner CE and Lydiate DJ (1999) Characterisation and mapping of resistance to turnip mosaic virus in oilseed rape (Brassica napus). Theoretical and Applied Genetics 99: 1149–1154
Walsh JA and Tomlinson JA (1985) Viruses infecting winter oilseed rape (Brassica napus ssp. oleifera) Annals of Applied Biology 107: 485–495
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Walsh, J.A., Rusholme, R.L., Hughes, S.L. et al. Different Classes of Resistance to Turnip Mosaic Virus in Brassica rapa . European Journal of Plant Pathology 108, 15–20 (2002). https://doi.org/10.1023/A:1013962911906
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DOI: https://doi.org/10.1023/A:1013962911906